Cortactin Antibody (2B5)

Western Blot: Cortactin Antibody (2B5) [H00002017-M01] - Analysis of CTTN expression in transfected 293T cell line by CTTN monoclonal antibody (M01), clone 2B5.Lane 1: CTTN transfected lysate (Predicted MW: 57.5 KDa).Lane 2: Non-transfected lysate.

Proximity Ligation Assay: Cortactin Antibody (2B5) [H00002017-M01] - Analysis of protein-protein interactions between SYK and CTTN. HeLa cells were stained with anti-SYK rabbit purified polyclonal 1:1200 and anti-CTTN mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Sandwich ELISA: Cortactin Antibody (2B5) [H00002017-M01] - Detection limit for recombinant GST tagged CTTN is 0.3 ng/ml as a capture antibody.

Product Discontinued

Cortactin Antibody (2B5) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: CTTN (NP_005222.2, 341 a.a. ~ 450 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EAVTSKTSNIRANFENLAKEKEQEDRRKAEAERAQRMAKERQEQEEARRKLEEQARAKTQTPPVSPAPQPTEERLPSSPVYEDAASFKAELSYRGPVSGTEPEPVYSMEA
• Specificity: CTTN - cortactin (2B5)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CTTN
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Proximity Ligation Assay
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Cortactin Antibody (2B5)

• 1110020L01Rik
• Amplaxin
• Cortactin
• CTTN
• ems1 sequence (mammary tumor and squamous cell carcinoma-associated (p80/85 srcsubstrate)
• EMS1
• EMS1amplaxin
• FLJ34459
• Oncogene EMS1
• src substrate cortactin

Background

This gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Two splice variants that encode different isoforms have been identified for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cortactin Antibody (H00002017-M01)(1)

Publication using H00002017-M01

• Halbert D, Domenyuk V, Spetzler D et al. Aptamers and uses thereof United States Patent Application US 9958448 B2 2018-01-01

Bioinformatics

• Gene Symbol: CTTN
• Entrez:
  • 2017(Human)
• Uniprot:
  • NP_005222(Human)