CORO2A Overexpression Lysate (Native)

Images

 
Western Blot: CORO2A Overexpression Lysate (Adult Normal) [NBL1-09400] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CORO2A.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

CORO2A Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC200403 and based on accession number NM_003389. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens coronin, actin binding protein, 2A (CORO2A), transcript variant 1, mRNA.
Gene
CORO2A

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
59.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for CORO2A

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for CORO2A Overexpression Lysate (Native)

  • CLIPINB
  • coronin, actin binding protein, 2A
  • coronin, actin-binding protein, 2A
  • coronin-like protein B
  • DKFZp686G19226
  • IR10coronin 2A
  • WD protein IR10
  • WD repeat-containing protein 2
  • WDR2coronin-2A
  • WD-repeat protein 2

Background

This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This protein contains 5 WD repeats, and has a structural similarity with actin-binding proteins: the D. discoideum coronin and the human p57 protein, suggesting that this protein may also be an actin-binding protein that regulates cell motility. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq]. Transcript Variant: This variant (1) contains a distinct 5' UTR transcribed from an alternate exon, as compared to variant 2. Variants 1 and 2 encode the same protein and contain the same 3' UTR. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol CORO2A