CORO1B Antibody (OTI3D10) [Janelia Fluor® 549]

• Reactivity: Hu, Mu, Rt
• Applications: Flow, ICC/IF
• Clone: OTI3D10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Janelia Fluor 549

CORO1B Antibody (OTI3D10) [Janelia Fluor® 549] Summary

• Immunogen: Full length recombinant protein of human CORO1B (NP_065174) produced in HEK293T cells.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CORO1B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for CORO1B Antibody (OTI3D10) [Janelia Fluor® 549]

• coronin, actin binding protein, 1B
• coronin, actin-binding protein, 1B
• coronin-1B
• coronin-2
• DKFZp762I166

Background

Coronin 2 is an F-actin binding protein that plays an important role in processes involving actin dynamics such as fibroblast migration, and phagocytosis. Coronin 2 influences actin dynamics by promoting actin depolymerization. It has been shown to interact with the Arp2/3 complex and inhibit Arp2/3-induced actin nucleation. Additionally, it can regulate motility by regulating the actin severing and disassembling activity of cofilin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CORO1B