COPS6 Antibody (OTI4E7) - Azide and BSA Free

Western Blot: COPS6 Antibody (OTI4E7) - Azide and BSA Free [NBP2-71648] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY COPS6.

Immunocytochemistry/Immunofluorescence: COPS6 Antibody (OTI4E7) - Azide and BSA Free [NBP2-71648] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY COPS6.

Immunohistochemistry: COPS6 Antibody (OTI4E7) - Azide and BSA Free [NBP2-71648] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: COPS6 Antibody (OTI4E7) - Azide and BSA Free [NBP2-71648] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: OTI4E7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

COPS6 Antibody (OTI4E7) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse COPS6 Antibody (OTI4E7) - Azide and BSA Free (NBP2-46333) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human recombinant protein fragment corresponding to amino acids 58-327 of human COPS6(NP_006824) produced in E.coli.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: COPS6
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
• Theoretical MW: 36 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for COPS6 Antibody (OTI4E7) - Azide and BSA Free

• BMP2B
• BMP2B1
• BMP4
• COP9 constitutive photomorphogenic homolog subunit 6 (Arabidopsis)
• COP9 signalosome complex subunit 6
• CSN6COP9 subunit 6 (MOV34 homolog, 34 kD)
• H_NH0506M12.12
• HVIP
• JAB1-containing signalosome subunit 6
• MOFC11
• MOV34 homolog
• MOV34 homolog, 34 kD
• MOV34-34KD
• SGN6
• Signalosome subunit 6
• Vpr-interacting protein

Background

COPS6 is encoded by this gene is one of the eight subunits of COP9 signalosome, a highly conserved protein complex that functions as an important regulator in multiple signaling pathways. The structure and function of COP9 signalosome is similar to that of the 19S regulatory particle of 26S proteasome. COP9 signalosome has been shown to interact with SCF-type E3 ubiquitin ligases and act as a positive regulator of E3 ubiquitin ligases. This protein belongs to translation initiation factor 3 (eIF3) superfamily. It is involved in the regulation of cell cycle and likely to be a cellular cofactor for HIV-1 accessory gene product Vpr. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: COPS6