COPG2 Antibody

Product Discontinued

COPG2 Antibody Summary

• Immunogen: Synthetic peptide corresponding to residues M(1) T T Y L E F I Q Q N E E R D G V R (18) C of rat COPII.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: COPG2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:200
  • Western Blot 2 ug/ml
• Application Notes: Useful in WB. WB: Detects an approx. 85 kDa protein representing COPII from PC-12 cell extract. May be useful in ICC and IP as reported in scientific literature.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS and 1 mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for COPG2 Antibody

• 2-COP
• coat protein, nonclathrin, gamma-2-cop
• coatomer protein complex, subunit gamma 2
• coatomer subunit gamma-2
• DKFZp761N09121
• FLJ11781
• Gamma-2-coat protein
• gamma-2-COP

Background

Coatomer proteins are involved in regulating transport between the endoplasmic reticulum (ER) and the Golgi complex and in intra-Golgi transport. There exist two Coatomer-Protein mechanisms (COPI and COPII) and although they have mechanistic parallels, they are molecularly distinct. The COPI coat is comprised of seven subunits (alpha-, beta-, beta'-, gamma-, delta-, epsilon-, and zeta-COP) in a complex called coatomer. Assembly of the coatomer (COPI) onto non-clathrin coated vesicles is regulated by ADP-ribosylation factor (ARF). Vesicle formation, budding, fusion, and disassembly is dependent on GDP-GTP exchange, COPI, and ARF. COPI has been shown to facilitate retrograde intracellular transport from the ER to the Golgi complex. By contrast, COPII facilitates anterograde transport between these subcellular organelles. COPII has been shown to be independently and selectively recruited to the ER relative to COPI subunits.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for COPG2 Antibody (NB300-521)(7)

Publications using NB300-521

• Brandon E, Szul T, Alvarez C, Grabski R, Benjamin R, Kawai R, Sztul E. On and off membrane dynamics of the endoplasmic reticulum-golgi tethering factor p115 in vivo. Mol Biol Cell;17(7):2996-3008. 2006-07-01 [PMID: 16624868] (ICC/IF)
• Ridsdale A, Denis M, Gougeon PY, Ngsee JK, Presley JF, Zha X. Cholesterol is required for efficient endoplasmic reticulum-to-Golgi transport of secretory membrane proteins. Mol Biol Cell;;17(4):1593-605. 2006-04-01 [PMID: 16452637] (ICC/IF)
• Altan-Bonnet N, Sougrat R, Liu W, Snapp EL, Ward T, Lippincott-Schwartz J. Golgi inheritance in mammalian cells is mediated through endoplasmic reticulum export activities. Mol Biol Cell;17(2):990-1005. 2006-02-01 [PMID: 16314396] (ICC/IF)
• Mardones GA, Snyder CM, Howell KE. Cis-Golgi matrix proteins move directly to endoplasmic reticulum exit sites by association with tubules. Mol Biol Cell;17(1):525-38. 2006-01-01 [PMID: 16251359] (ICC/IF)
• Nakajima K, Hirose H, Taniguchi M et al. Involvement of BNIP1 in apoptosis and endoplasmic reticulum membrane fusion. EMBO J 2004-08-18 [PMID: 15272311] (IP)
• Gusarova V, Brodsky JL, Fisher EA. Apolipoprotein B100 exit from the endoplasmic reticulum (ER) is COPII-dependent, and its lipidation to very low density lipoprotein occurs post-ER. J Biol Chem;278(48):48051-8. 2003-11-28 [PMID: 12960170] (IP)
• Kondylis V, Rabouille C. A novel role for dp115 in the organization of tER sites in Drosophila. J Cell Biol;162(2):185-98. 2003-07-21 [PMID: 12876273] (ICC/IF)

Bioinformatics

• Gene Symbol: COPG2
• Entrez:
  • 26958(Human)
  • 54160(Mouse)
  • 58847(Rat)
• Uniprot:
  • B5DFC3(Rat)