COP9 Antibody - BSA Free

Western Blot: COP9 Antibody [NB100-365] - Detection of Human CSN3 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CSN3 antibody NB100-365 used for WB at 0.05 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Western Blot: COP9 Antibody [NB100-365] - Whole cell lysate (50 ug - E; 10 ug - T) from mock transfected (E) or CSN3 transfected (T) HEK293T cells. Antibody used at the indicated concentration.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

COP9 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit COP9 Antibody - BSA Free (NB100-365) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residues 325 and 375 of human COP9 constitutive photomorphogenic homolog subunit 3 using the numbering given in entry NP_003644.2 (GeneID 8533).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: COPS3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:2000-1:20000
• Application Notes: By Western blot, this antibody recognizes a band at ~40 kDa, representing CSN3 protein. *The investigator should determine the optimal working dilution for a specific application.
• Control:

  COP9 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for COP9 Antibody - BSA Free

• COP9 constitutive photomorphogenic homolog subunit 3 (Arabidopsis)
• COP9 signalosome complex subunit 3
• CSN3COP9 complex subunit 3
• JAB1-containing signalosome subunit 3
• SGN3COP9 (constitutive photomorphogenic, Arabidopsis, homolog) subunit 3
• Signalosome subunit 3

Background

COP9 signalosome is a conserved protein composed of eight subunits. Individual subunits of the complex have been linked to various signal transduction pathways leading to gene expression and cell cycle control. CSN3 encodes the third subunit of COP9 signalosome. CSN3 is important for maintaining the integrity of the COP9 signalosome and crucial for the survival of epiblast cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: COPS3
• Entrez:
  • 8533(Human)
• Uniprot:
  • NP_003644.2(Human)