COP1 Antibody (1E4)

Images

 
Western Blot: COP1 Antibody (1E4) [H00064326-M01] - RFWD2 monoclonal antibody (M01), clone 1E4 Analysis of RFWD2 expression in NIH/3T3.
Immunocytochemistry/ Immunofluorescence: COP1 Antibody (1E4) [H00064326-M01] - Analysis of monoclonal antibody to RFWD2 on NIH/3T3 cell. Antibody concentration 10 ug/ml.
Western Blot: COP1 Antibody (1E4) [H00064326-M01] - RFWD2 monoclonal antibody (M01), clone 1E4. Analysis of RFWD2 expression in A-431.
Western Blot: COP1 Antibody (1E4) [H00064326-M01] - RFWD2 monoclonal antibody (M01), clone 1E4. Analysis of RFWD2 expression in Jurkat.
Western Blot: COP1 Antibody (1E4) [H00064326-M01] - RFWD2 monoclonal antibody (M01), clone 1E4. Analysis of RFWD2 expression in K-562.
Sandwich ELISA: COP1 Antibody (1E4) [H00064326-M01] - Detection limit for recombinant GST tagged RFWD2 is approximately 0.1ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
1E4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

COP1 Antibody (1E4) Summary

Immunogen
RFWD2 (NP_071902, 632 a.a. ~ 732 a.a) partial recombinant protein with GST tag.CLRSFKGHINEKNFVGLASNGDYIACGSENNSLYLYYKGLSKTLLTFKFDTVKSVLDKDRKEDDTNEFVSAVCWRALPDGESNVLIAANSQGTIKVLELV
Specificity
RFWD2 - ring finger and WD repeat domain 2
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
RFWD2
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for COP1 Antibody (1E4)

  • Constitutive photomorphogenesis protein 1 homolog
  • constitutive photomorphogenic protein (COP1)
  • COP1RNF200FLJ10416
  • E3 ubiquitin-protein ligase RFWD2
  • EC 6.3.2.-
  • hCOP1
  • putative ubiquitin ligase COP1
  • ring finger and WD repeat domain 2
  • RING finger and WD repeat domain protein 2
  • RING finger protein 200

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for COP1 Antibody (H00064326-M01) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for COP1 Antibody (H00064326-M01). (Showing 1 - 1 of 1 FAQ).

  1. Could you let us know whether this antibody has been mentioned in a publication so that we can look at those images? Moreover, could you please tell us whether the immunoblot presented on you website shows the labeling of the endogenous RFWD2 from 3T3 cells or whether a lysate of cells transfected with an RFWD2 expression vector has been used for blotting?
    • This product has not yet been mentioned in any publications that we are aware of. And in regards to the WB image, this image was generated with 3T3 cells showing the staining of endogenous COP1 / RFWD2.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RFWD2
Entrez
OMIM
Uniprot