SIPA1 Antibody - BSA Free

Western Blot: SIPA1 Antibody [NBP2-20369] - Western Blot analysis of SIPA1 expression in transfected 293T cell line by SIPA1 polyclonal antibody. A: Non-transfected lysate. B: SIPA1 transfected lysate. 7. 5% SDS PAGE gel, diluted at 1:500.

Immunocytochemistry/Immunofluorescence: SIPA1 Antibody [NBP2-20369] - Analysis of methanol-fixed mouse ESC, using SIPA1 antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: SIPA1 Antibody [NBP2-20369] - Paraffin-embedded HBL435 xenograft. SIPA1 antibody [C3], C-term dilution: 1:500.

Immunohistochemistry-Paraffin: SIPA1 Antibody [NBP2-20369] - Paraffin-embedded Cal27 xenograft. SIPA1 antibody [C3], C-term dilution: 1:500.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SIPA1 Antibody - BSA Free Summary

• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human SIPA1. The exact sequence is proprietary.
• Predicted Species: Rat (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SIPA1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 112 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 40% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for SIPA1 Antibody - BSA Free

• GTPase-activating protein Spa-1
• MGC102688
• MGC17037
• p130 SPA-1
• signal-induced proliferation-associated 1
• signal-induced proliferation-associated protein 1
• sipa-1
• SPA1signal-induced proliferation-associated gene 1

Background

The product of this gene is a mitogen induced GTPase activating protein (GAP). It exhibits a specific GAP activity for Ras-related regulatory proteins Rap1 and Rap2, but not for Ran or other small GTPases. This protein may also hamper mitogen-induced cell cycle progression when abnormally or prematurely expressed. It is localized to the perinuclear region. Two alternatively spliced variants encoding the same isoform have been characterized to date. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: SIPA1
• Uniprot:
  • Q96FS4(Human)