Contactin-2/TAG1 Antibody [Alexa Fluor® 532]

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 532

Contactin-2/TAG1 Antibody [Alexa Fluor® 532] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Contactin‑2/TAG1
  Leu29-Asn1012
  Accession # Q02246
• Specificity: Detects human Contactin‑2/TAG1 in direct ELISAs. Detects human, mouse, and rat Contactin‑2/TAG1 in Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) Contactin-3, rhContactin-4, rhContactin-5 and rhContact
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Contactin-2/TAG1 Antibody [Alexa Fluor® 532]

• Axonal glycoprotein TAG-1
• axonin-1 cell adhesion molecule
• Axonin-1
• AXT
• CNTN2
• contactin 2 (axonal)
• contactin 2 (transiently expressed)
• Contactin2
• Contactin-2
• DKFZp781D102
• FLJ37193
• MGC157722
• TAG1
• TAG-1
• TAX
• TAX1
• TAX-1
• TAX1FLJ42746
• Transient axonal glycoprotein 1
• transiently-expressed axonal glycoprotein

Background

Contactin-2 (CNTN2), also called TAG-1 (transient axonal glycoprotein), TAX1 (transiently-expressed axonal glycoprotein), or axonin-1, is a 135 kDa glycosyl‑phosphatidylinositol (GPI)- anchored cell adhesion molecule that belongs to the contactin subfamily within the immunoglobulin (Ig) protein superfamily (1‑3). Human Contactin-2 cDNA encodes a 28 amino acid (aa) signal peptide, a 984 aa mature secreted protein with six Ig-like domains followed by four fibronectin type III‑like repeats, and a 28 aa C-terminal GPI anchor pro-sequence. GPI-specific phospholipase activity can release soluble, active Contactin-2 from the membrane (2). Mature human Contactin-2 shares approximately 93%, 93% and 75% aa sequence identity with human, rat and chicken Contactin-2, respectively. During development, Contactin-2 is expressed by a subset of neuronal populations in the central nervous system (CNS) and peripheral nervous system (PNS), particularly during initial phases of axon outgrowth (3‑5). Both the 135 kDa form and a 90 kDa form are also upregulated in response to CNS injury in the adult (6). Data support a role for Contactin-2 in axon pathfinding, neurite outgrowth and adhesion, especially in the CNS (3‑6). In mature myelinated fibers, Contactin‑2 is expressed by oligodendrocytes and Schwann cells, which are myelinating glial cells of the CNS and PNS, respectively (7, 8). It is enriched in the juxtaparanodal regions, where it recruits caspr2 (Contactin‑associated protein 2), a transmembrane neurexin involved in cell adhesion and intercellular communication (7‑10). The axonal Contactin‑2 interacts in cis with caspr2, and in trans with another Contactin‑2 on the glial membrane (8). This ternary complex is required for the accumulation and organization of K⁺ channels in the juxtaparanodes (9).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.