Contactin-1 Antibody (2332B) [Unconjugated]

Western blot shows lysates of human brain tissue and A549 human lung carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human Contactin-1 Monoclonal Antibody (Catalog # MAB9041) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Contactin-1 at approximately 135 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clone: 2332B
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Contactin-1 Antibody (2332B) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Human embryonic kidney cell, HEK293-derived human Contactin-1
  Met1-Ser993
  Accession # Q12860
• Specificity: Detects human Contactin-1 in direct ELISAs.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Contactin-1 Antibody (2332B) [Unconjugated]

• CNTN1
• contactin 1
• Contactin1
• Contactin-1
• F11
• F3
• F3cam
• Glycoprotein gp135
• GP135
• Neural cell surface protein F3

Background

Contactin-1 is a GPI-anchored adhesion protein. It is preferentially expressed in neuronal tissues and interacts with Nr-CAM and Tenascin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Contactin-1 Antibody (MAB9041)(1)

Publication using MAB9041

• Monika Pietrowska, Aneta Zebrowska, Marta Gawin, Lukasz Marczak, Priyanka Sharma, Sujan Mondal et al. Proteomic profile of melanoma cell‐derived small extracellular vesicles in patients’ plasma: a potential correlate of melanoma progression Journal of Extracellular Vesicles 2021-02-11 [PMID: 33613873] (Western Blot, Human)

Bioinformatics

• Uniprot:
  • Q12860()