Connexin 45/GJC1 Antibody (5B9.2) - BSA Free

Immunocytochemistry/Immunofluorescence: Connexin 45/GJC1 Antibody (5B9.2) - BSA Free [NBP2-29754] - Mouse anti-Connexin 45 staining of Cx45 transfected rat C6 cells.

• Reactivity: Hu, Rt, Rb, Mu, Ca
• Applications: WB, ICC/IF
• Clone: 5B9.2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Connexin 45/GJC1 Antibody (5B9.2) - BSA Free Summary

• Description: Novus Biologicals Mouse Connexin 45/GJC1 Antibody (5B9.2) - BSA Free (NBP2-29754) is a monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The peptide sequence used for the immunogen was H-Gln-Ala-Tyr-Ser-His-Gln-Asn-Asn-Pro-His-Gly-Pro-Arg-Glu-Cys-OH conjugated to KLH, corresponding to amino acids 354-377 of human connexin 45.
• Predicted Species: Mouse (92%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: GJC1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Western Blot 1:100-1:2000
• Application Notes: ImmunBlotting (~46 kDa)
• Theoretical MW: 46 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Due to sequence homologies, this antibody is also expected to react with canine (100% homology) and mouse (92% homology) Connexin 45. Also confirmed to work on rat C6 cells. Others not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 20mM Phosphate (pH 7.6) and 0.25M NaCl
• Preservative: 0.1% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Connexin 45/GJC1 Antibody (5B9.2) - BSA Free

• Connexin 45
• connexin-45
• CX45
• DKFZp686P0738
• Gap junction alpha-7 protein
• gap junction gamma-1 protein
• gap junction protein, alpha 7, 45kDa
• gap junction protein, gamma 1, 45kDa
• GJA7
• GJC1

Background

One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. A connexon is composed of a hexamer of connexins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GJC1
• Uniprot:
  • P36383(Human)