Connexin 45/GJC1 Antibody (5B9.2) [Janelia Fluor® 646]

• Reactivity: Hu, Rt, Rb, Mu, Ca
• Applications: WB, ICC/IF
• Clone: 5B9.2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Janelia Fluor 646

Connexin 45/GJC1 Antibody (5B9.2) [Janelia Fluor® 646] Summary

• Immunogen: The peptide sequence used for the immunogen was H-Gln-Ala-Tyr-Ser-His-Gln-Asn-Asn-Pro-His-Gly-Pro-Arg-Glu-Cys-OH conjugated to KLH, corresponding to amino acids 354-377 of human connexin 45.
• Predicted Species: Mouse (92%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: GJC1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Due to sequence homologies, this antibody is also expected to react with canine (100% homology) and mouse (92% homology) Connexin 45. Also confirmed to work on rat C6 cells. Others not tested.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Connexin 45/GJC1 Antibody (5B9.2) [Janelia Fluor® 646]

• Connexin 45
• connexin-45
• CX45
• DKFZp686P0738
• Gap junction alpha-7 protein
• gap junction gamma-1 protein
• gap junction protein, alpha 7, 45kDa
• gap junction protein, gamma 1, 45kDa
• GJA7
• GJC1

Background

One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. A connexon is composed of a hexamer of connexins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GJC1