Connexin 43/GJA1 Antibody (M496)

Western Blot: Connexin 43/GJA1 Antibody (M496) [NBP3-23240] - Western blot analysis of Connexin 43/GJA1 expression in adult mouse brain (lanes 1-3). The blots were probed with mouse monoclonal anti-Connexin 43/GJA1 at 1:250 (lane 1), 1:500 (lane 2), and 1:1000 (lane 3).

• Reactivity: Hu, Mu, Rt, Ch
• Applications: WB, ICC/IF
• Clone: M496
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Connexin 43/GJA1 Antibody (M496) Summary

• Description: Stable for at least 1 year at -20°C.
• Immunogen: Clone M496 was generated from a peptide fragment in the C-terminal region of human Connexin 43/GJA1. This sequence is highly conserved in mouse and rat Connexin 43/GJA1. [UniProt# P17302]
• Specificity: The antibody detects a 43 kDa protein corresponding to the apparent molecular mass of Connexin 43/GJA1 on SDS-PAGE immunoblots of mouse brain. The antibody is also useful for immunofluorescent labeling of gap junctions in cells.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: GJA1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:1000
• Theoretical MW: 43 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS, 1 mg/ml BSA and 50% glycerol
• Preservative: 0.05% Sodium Azide
• Purity: Antigen Affinity-purified

Alternate Names for Connexin 43/GJA1 Antibody (M496)

• Connexin 43
• connexin-43
• CX43
• CX43gap junction protein, alpha-like
• DFNB38
• Gap junction 43 kDa heart protein
• gap junction alpha-1 protein
• gap junction protein, alpha 1, 43kDa (connexin 43)
• gap junction protein, alpha 1, 43kDa
• GJA1
• GJAL
• HSS
• ODD
• ODDD
• ODOD
• SDTY3

Background

Connexin 43/GJA1 is a member of the connexin gene family and a component of gap junctions. Gap junctions are composed of arrays of intercellular channels and provide a route for the diffusion of materials of low molecular weight from cell to cell. GJA1 is the major cardiac gap junction protein, which is thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. GJA1 is targeted by several protein kinases that regulate myocardial cell cell coupling. A related intronless GJA1 pseudogene, GJA1P, has been mapped to chromosome 5.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GJA1