Connexin 40/GJA5 Antibody

Immunohistochemistry-Paraffin: Connexin 40/GJA5 Antibody [NBP1-46568] - IHC on paraffin sections of rat kidney tissue using Rabbit antibody to Connexin 40. HIER: 1 mM EDTA, pH 8 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer from Leica following manufactures instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer. Sections were counterstained with Harris Hematoxylin.

Immunohistochemistry-Paraffin: Connexin 40/GJA5 Antibody [NBP1-46568] - IHC on paraffin sections of rat kidney tissue using Rabbit antibody to Connexin 40. HIER: 1 mM EDTA, pH 8 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer from Leica following manufactures instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer. Sections were counterstained with Harris Hematoxylin.

• Reactivity: Mu, Rt
• Applications: WB, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: LYOPH

Connexin 40/GJA5 Antibody Summary

• Immunogen: A synthetic peptide from 2d cytoplasmic domain of human 2d cytoplasmic domain of Connexin-40 (CX40, CXA5) conjugated to an immunogenic carrier protein was used as the antigen.
• Specificity: Specific for Connexin-40.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GJA5
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:300-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin
• Application Notes: Western Blot. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Although not tested this antibody may work in Immunohistochemistry-Paraffin/Frozen.

Reactivity Notes

Other species not yet tested but expected to work in rat.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Unpurified
• Reconstitution Instructions: Reconstitute with 0.1 ml sterilized water to desired concentration.

Alternate Names for Connexin 40/GJA5 Antibody

• alpha 5, 40kD (connexin 40)
• ATFB11
• Connexin 40
• connexin-40
• CX40
• gap junction alpha-5 protein
• gap junction protein, alpha 5, 40kDa (connexin 40)
• gap junction protein, alpha 5, 40kDa
• GJA5
• MGC11185

Background

One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. A connexon is composed of a hexamer of connexins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GJA5
• Entrez:
  • 2702(Human)
  • 14613(Mouse)
  • 50563(Rat)