Complement Factor H-related 4/CFHR4 Antibody [Unconjugated]

Western blot shows lysates of human plasma. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human Complement Factor H-related 4/CFHR4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5980) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Complement Factor H-related 4/CFHR4 at approximately 86 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Complement Factor H-related 4/CFHR4 Antibody [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Complement Factor H-related 4/CFHR4
  Arg41-Glu331 (Ser79Leu)
  Accession # Q92496
• Specificity: Detects human Complement Factor H-related 4/CFHR4 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross‑reactivity with recombinant human (rh) CFHR1, rhCFHR2, and rhCFHR5 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: CFHR4
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Complement Factor H-related 4/CFHR4 Antibody [Unconjugated]

• complement factor H-related protein 4
• CFHL4
• CFHR4
• Complement Factor Hrelated 4
• Complement Factor H-related 4
• FHR4
• FHR-4

Background

FHR4 (Complement factor H-related protein 4; also FHR4B and CFHR4) is a 45 kDa glycoprotein member of the factor H-related protein family. It is synthesized by hepatocytes and circulates in plasma. FHR4(B) binds to C3b and C3d. It also binds to native (pentameric) C-reactive protein (pCRP), and is suggested to promote pCRP binding to necrotic cells surfaces, thus promoting cell clearance. Mature human CFHR is 312 amino acids (aa) in length. It contains five Sushi/SCR domains, the first of which (aa 23-85) is associated with pCRP binding. There is a circulating, 86 kDa alternative splice form termed FHR4A that shows an insertion of 247 aa after Asp92. This is considered the dominant isoform, and appears to show the same activity as CFHR. Over aa 41-331, human CFHR shares 61% aa identity with a related molecule found in rat.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Complement Factor H-related 4/CFHR4 Antibody (AF5980)(1)

Publication using AF5980

• Zouache, MA;Richards, BT;Pappas, CM;Anstadt, RA;Liu, J;Corsetti, T;Matthews, S;Seager, NA;Schmitz-Valckenberg, S;Fleckenstein, M;Hubbard, WC;Thomas, J;Hageman, JL;Williams, BL;Hageman, GS; Levels of complement factor H-related 4 protein do not influence susceptibility to age-related macular degeneration or its course of progression Nature communications 2024-01-10 [PMID: 38200010] (ELISA Detection, Human)

Bioinformatics

• Gene Symbol: CFHR4
• Uniprot:
  • Q92496()