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Home » Complement Factor H-related 1/CFHR1/CFHL1 » Complement Factor H-related 1/CFHR1/CFHL1 ELISA Kits » Human Complement Factor H-related 1/CFHR1/CFHL1 ELISA Kit (Colorimetric)

Human Complement Factor H-related 1/CFHR1/CFHL1 ELISA Kit (Colorimetric)

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Human Complement Factor H-related 1/CFHR1/CFHL1 ELISA Kit (Colorimetric) - Standard Curve Reference
Human Complement Factor H-related 1/CFHR1/CFHL1 ELISA Kit (Colorimetric) - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids.
Standard Curve Range
0.63 - 40 ng/mL
Sensitivity
0.38 ng/mL

Order Details

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Human Complement Factor H-related 1/CFHR1/CFHL1 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human CFHR1 in samples. No significant cross-reactivity or interference between Human CFHR1 and analogues was observed.
Standard Curve Range
0.63 - 40 ng/mL
Sensitivity
0.38 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 4.87%
Intra-Assay
CV% < 5.54%
Spike Recovery
87-105%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
CFHR1

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Certificate of Analysis
  3. Concentrated Biotinylated Detection Ab (100×)
  4. Concentrated HRP Conjugate (100×)
  5. Concentrated Wash Buffer (25×)
  6. HRP Conjugate Diluent
  7. Micro ELISA Plate(Dismountable)
  8. Plate Sealer
  9. Product Description
  10. Reference Standard & Sample Diluent
  11. Reference Standard
  12. Stop Solution
  13. Substrate Reagent

Alternate Names for Human Complement Factor H-related 1/CFHR1/CFHL1 ELISA Kit (Colorimetric)

  • BTA
  • CFHL1FHR-1
  • CFHLCFHR1P
  • CFHR1
  • complement factor H-related 1 pseudogene
  • Complement Factor Hrelated 1
  • Complement Factor H-related 1
  • complement factor H-related protein 1
  • FHR1
  • FHR1CFHL1P
  • H factor (complement)-like 1
  • H factor (complement)-like 2
  • H factor-like protein 1
  • H36-1
  • H36-2
  • H-factor-like 1
  • HFL1H36
  • HFL2
  • MGC104329

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Complement Factor&amp;#160;H was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human Complement Factor&amp;#160;H Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4779) at 10&amp;#160;&amp;micro;g/mL overnight at 4&amp;#160;&amp;deg;C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell &amp; Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Western Blot: FHL1 Antibody (OTI3G7) - Azide and BSA Free [NBP2-70739] - Analysis of extracts (35ug) from 9 different cell lines by using anti-FHL1 monoclonal antibody.
Western Blot: FHL1 Antibody (OTI3G7) - Azide and BSA Free [NBP2-70739] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FHL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FHL1.
Flow Cytometry: FHL1 Antibody (OTI3G7) - Azide and BSA Free [NBP2-70739] - Analysis of Hela cells, using anti-FHL1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Species: Hu, Mu, Rt
Applications: CyTOF-ready, Flow, ICC/IF, WB
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Western Blot: Complement Factor H-related 3/CFHR3 Antibody [NBP2-92515] - Analysis of extracts of various cell lines, using Complement Factor H-related 3/CFHR3 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
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Flow Cytometry: Complement C3 Antibody (11H9) [NB200-540] - Left panel: FMO, Middle panel: No primary antibody control, Right panel: sample. Day 6 murine mammary tumors processed and stained for analysis with flow cytometry. The C3b+ population of CD45+ cells is what the gate in each sample is exhibiting. WB image submitted by a verified customer review.
Immunocytochemistry/Immunofluorescence: Complement C3 Antibody (11H9) [NB200-540] - C3 protein fragments deposited on kidney cells of MPL-lpr mouse. Staining with antibody 11H9. Glomerular staining pattern. Fixation in 4% paraformaldehyde in PBS pH 7.4. Vibratome sections of 4 um. Pretreated with 3% hydrogen peroxide for 20 min to quench endogenous peroxidases. Microwaved in antigen unmasking solution for 2-5 minutes as antigen retrieval.
Immunohistochemistry-Paraffin: Complement C3 Antibody (11H9) [NB200-540] - Complement C3 protein in a FFPE tissue section of mouse lymph node using 1:100 dilution of Complement C3 antibody (clone 11H9) NB200-540. This representative photomicrograph shows a membrane-cytoplasmic immunopositivity in non-germinal center cells, and few cells developed an intense staining for this target protein.
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Immunocytochemistry/Immunofluorescence: Fumarase Antibody [NBP1-31336] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Fumarate hydratase protein stained by Fumarate hydratase antibody diluted at 1:500.Blue: Hoechst 33342 staining.
Western Blot: Fumarase Antibody [NBP1-31336] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Fumarate hydratase antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: Fumarase Antibody [NBP1-31336] - Representative western blots showing protein expression of fumarase and key enzymes in OXPHOS. VDAC was used as a loading control (n = 3). Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/srep22788) licensed under a CC-BY licence.
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Immunohistochemistry: LDL R Antibody (C7) [NBP1-78159] - Analysis of an FFPE tissue section of human liver using LDL Receptor antibody (clone C7) with HRP-DAB based detection and hematoxylin counterstaining.
Immunocytochemistry/Immunofluorescence: LDL R Antibody (C7) [NBP1-78159] - Image from a customer review on porcine intestinal epithelial cells IPEC-J2.
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Western Blot: Complement Factor B Antibody [NBP1-89985] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
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Western Blot: Complement Factor H-related 5/CFHR5 Antibody (3A10A5) [NBP2-52441] - Analysis using CFHR5 mAb against HEK293 (1) and CFHR5 (AA: 344-569)-hIgGFc transfected HEK293 (2) cell lysate.
ELISA: Complement Factor H-related 5/CFHR5 Antibody (3A10A5) [NBP2-52441] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng).
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Western Blot: Plasminogen Antibody [NB600-930] - Detection of Plasminogen under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of 1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody. Detection shown was using Dylight 649 conjugated Donkey anti goat 1 hr RT.
Western Blot: Plasminogen Antibody [NB600-930] - Lane 1: Plasminogen. Lane 2: None. Load: 50 ng per lane. Primary antibody: Plasminogen primary antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase goat secondary antibody at 1:40,000 for 60 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 91 kDa, 91 kDa for Plasminogen. Other band(s): None.
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Immunohistochemistry-Paraffin: C4 binding protein A Antibody [NBP1-88263] - Staining of human liver using Anti-C4BPA antibody.
Immunohistochemistry-Paraffin: C4 binding protein A Antibody [NBP1-88263] - Staining of human lung using Anti-C4BPA antibody.
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Immunohistochemistry-Paraffin: AMD1 Antibody [NBP2-97180] - Immunochemical staining of human AMD1 in human liver with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.
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Human Complement Factor H-related 1/CFHR1/CFHL1 ELISA Kit (Colorimetric)
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Applications: ELISA

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  Human Complement Factor H-related 1/CFHR1/CFHL1 ELISA Kit (Colorimetric) Manual
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Bioinformatics

Gene Symbol CFHR1