Complement Component C2 Antibody (5C3) - BSA Free

Western Blot: Complement Component C2 Antibody (5C3) - BSA Free [NBP2-23549] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil

Immunohistochemistry: Complement Component C2 Antibody (5C3) - BSA Free [NBP2-23549] - Staining of human kidney shows strong positivity in extracellular material.

Product Discontinued

Complement Component C2 Antibody (5C3) - BSA Free Summary

• Immunogen: Complement component C2 isolated from human plasma adsorbed onto aluminum hydroxide gel
• Epitope: Epitope specificity differs from that of Clone 05 and Clone 08 as determined by inhibition ELISA.
• Specificity: Clone 5C3 is specific for C2 from human plasma/serum. Cross-reactivity to C2 from other species has not been tested.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: C2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:4000
  • Western Blot 1:100-1:2000
• Application Notes: Clone 5C3 reacts with human C2 in both native and denatured forms. In Western blotting after SDS-PAGE, Clone 5C3 reacts with C2 and a subfraction of C2 believed to be C2b, both under reducing and non-reducing conditions. In ELISA, strong reaction is seen with C2 directly coated onto the microtiter well.

Reactivity Notes

Human

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 10mM Phosphate (pH 7.4) and 0.5M NaCl
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Complement Component C2 Antibody (5C3) - BSA Free

• C2
• C3/C5 convertase
• CO2
• complement C2
• complement component 2
• Complement Component C2
• DKFZp779M0311
• EC 3.4.21
• EC 3.4.21.43

Background

Complement component C2 is a component of the classical complement pathway and is involved in activation of C3 and C5. C2 consists of a single polypeptide chain with a molecular mass of 102 kDa .

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: C2