Complement Component C1r Antibody [Alexa Fluor® 594]

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 594

Complement Component C1r Antibody [Alexa Fluor® 594] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Complement Component C1r
  Ser18-Asp705
  Accession # NP_001724
• Specificity: Detects human Complement Component C1r in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human Complement Component C1s is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Complement Component C1r Antibody [Alexa Fluor® 594]

• C1r
• complement C1r subcomponent
• Complement component 1 subcomponent r
• complement component 1, r subcomponent
• Complement Component C1r
• EC 3.4.21
• EC 3.4.21.41

Background

The classical complement pathway plays a major role in innate immunity against infection. This pathway is triggered by C1, a multimolecular complex composed of the recognition protein C1q and two serine proteases, C1r and C1s. Following the C1q recognition, C1r is autoactivated, and in turn activates C1s, which cleaves C4 and C2, the C1 substrates (1). Both C1r and C1s activation involve cleavage of a specific Arg-Ile bond, converting single-chain proenzymes into active proteases of disulfide bond-linked chains (A and B) (2). The A chains contain multiple domains in the order of CUB1-EGF-CUB2-CCP1-CCP2-Activation Peptide. The B chains contain the serine protease catalytic domain. The full-length (amino acid residues 1‑705) of human C1r was expressed, which had the Leu152 natural variant (3). The purified protein corresponded to the processed active form, with A and B chains starting at residue Ser18 and Ile464, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.