Human Complement C3a ELISA Kit (Colorimetric)

Product Discontinued

Human Complement C3a ELISA Kit (Colorimetric) Summary

• Standard Curve Range: 0.625 to 40 ng/mL
• Sensitivity: 0.6 ng/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 7.50%
• Intra-Assay: CV% < 4.80%
• Spike Recovery: 97%
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: C3

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: C3 (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of human complement C3 in urine, milk, saliva, and cell culture supernatants.

Reactivity Notes

Human

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Human Complement C3a ELISA Kit (Colorimetric)

• Acylation Stimulating Protein
• acylation-stimulating protein cleavage product
• AHUS5
• ARMD9
• ASP
• C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1
• C3
• C3a anaphylatoxin
• C3a
• C3adesArg
• C3b
• C3bc
• C3-beta-c
• Complement C3 alpha chain
• Complement C3 beta chain
• complement C3
• Complement C3b alpha' chain
• Complement C3c alpha' chain fragment 1
• Complement C3c alpha' chain fragment 2
• Complement C3d fragment
• Complement C3dg fragment
• Complement C3f fragment
• Complement C3g fragment
• complement component 3
• complement component C3
• complement component C3a
• complement component C3b
• CPAMD1
• EC 3.4.21.43
• epididymis secretory sperm binding protein Li 62p
• HEL-S-62p
• prepro-C3

Background

The complement system, or complement cascade, is a part of the innate immune system that assists in defense against pathogens (1). Complement C3, also called C3 or C3 protein, is one of nine complement proteins and is the main component of the complement system which is composed of over 30 soluble and membrane-bound proteins (1). C3 is cleaved by C3 convertase into two fragments, the smaller C3a and larger C3b (1,2). Human C3a is 77 amino acids (aa) in length and has a theoretical molecular weight of ~9 kDa (2). Structurally, complement C3a consists of four anti-parallel helical structures with three disulfide bridges (2). The C3a fragment binds the G-protein coupled receptor (GPCR) C3a (C3aR) (2,3). Functionally, C3a plays an important role in regulating both adaptive immunity and innate immunity (1-3).

The C3a/C3aR pathway is often referred to as a "double-edged sword" because it has both anti-inflammatory and pro-inflammatory functions (2,3). For instance, its anti-inflammatory role includes pathogen elimination and reduced interferon-gamma (IFN-gamma) expression and cytotoxicity in natural killer cells (2). By contrast, complement C3a is an anaphylatoxin and plays a role in inflammation by prompting histamine release from mast cells and increasing permeability of the vascular system (2-4). The C3a/C3aR pathway has also been indicated in a number of diseases including Alzheimer's disease, asthma, and kidney disease (3). Patients with various kidney diseases tend to show higher levels of complement C3a in the plasma and urine (3).

References

1. Mathern, D. R., & Heeger, P. S. (2015). Molecules Great and Small: The Complement System. Clinical Journal of the American Society of Nephrology: CJASN. https://doi.org/10.2215/CJN.06230614

2. Gao, S., Cui, Z., & Zhao, M. H. (2020). The Complement C3a and C3a Receptor Pathway in Kidney Diseases. Frontiers in Immunology. https://doi.org/10.3389/fimmu.2020.01875

3. Ricklin, D., Reis, E. S., Mastellos, D. C., Gros, P., & Lambris, J. D. (2016). Complement component C3 - The "Swiss Army Knife" of innate immunity and host defense. Immunological Reviews. https://doi.org/10.1111/imr.12500

4. Haddad, A., & Wilson, A. M. (2020). Biochemistry, Complement. In StatPearls. StatPearls Publishing.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Complement C3a ELISA Kit (KA1020)(5)

Publications using KA1020

• Han R, Hu S, Qin W, et al. C3a and suPAR drive versican V1 expression in tubular cells of focal segmental glomerulosclerosis JCI Insight 2020-04-04 [PMID: 30944246] (ELISA, Human)
• Signorile PG, Baldi A. Supporting evidences for potential biomarkers of endometriosis detected in peripheral blood. Data in Brief. 2015-11-17 [PMID: 26759817]
• Ramos-Casals M, Brito-Zeron P, Nardi N et al. Successful treatment of severe Raynaud's phenomenon with bosentan in four patients with systemic sclerosis. Rheumatology (Oxford) 2004-11-01 [PMID: 15502001]
• Sacks S, Zhou W. The effect of locally synthesised complement on acute renal allograft rejection. J Mol Med 2003-06-25 [PMID: 12827269]
• Sahu A, Lambris JD. Structure and biology of complement protein C3, a connecting link between innate and acquired immunity. Immunol Rev 2001-04-01 [PMID: 11414361]

FAQs for Complement C3a ELISA Kit (KA1020). (Showing 1 - 1 of 1 FAQs).

1. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Bioinformatics

• Gene Symbol: C3