Common gamma Chain/IL-2 R gamma Antibody (633162) [CoraFluor™ 1]

• Reactivity: Hu
• Applications: Flow, B/N, CyTOF-ready
• Clone: 633162
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: CoraFluor 1

Common gamma Chain/IL-2 R gamma Antibody (633162) [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
• Immunogen: S. frugiperda insect ovarian cell line Sf21-derived recombinant human Common gamma Chain/IL-2 gamma
  Leu23-Asn254
  Accession # P31785
• Specificity: Detects human Common gamma Chain/IL-2 R gamma in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-2 R alpha , rhIL-2 R beta , rhIL-15 R alpha , recombinant mouse IL-2 R gamma , or recombinant canine IL-2 R alpha is observed. In Western blots, approximately 80% cross-reactivity with recombinant human IL-2 R beta , 50% cross-reactivity with rhIL-15 R alpha and recombinant mouse IL-2 R alpha , and no cross-reactivity with rhIL-2 R alpha is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL2RG
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry
  • Neutralization
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A or G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for Common gamma Chain/IL-2 R gamma Antibody (633162) [CoraFluor™ 1]

• CD132 antigen
• CD132
• CIDX
• combined immunodeficiency, X-linked
• common cytokine receptor gamma chain
• Common gamma Chain
• common gamma-chain
• cytokine receptor common subunit gamma
• gamma(c)
• IL-2 R gamma
• IL-2 receptor subunit gamma
• IL2R gamma
• IL-2R subunit gamma
• IL2RG
• IL-2RG
• IMD4
• interleukin 2 receptor, gamma
• Interleukin-2 receptor subunit gamma
• P64
• SCIDX
• SCIDX1
• severe combined immunodeficiency

Background

The interleukin 2 (IL2) receptor gamma chain (IL2RG), an important signalling component of many interleukin receptors(IL2,IL4,IL7,IL9, and IL15), is thus referred to as the common gamma chain. Mutations in this X-chromosome-linked genecause X-linked severe combined immunodeficiency (XSCID). (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL2RG