Recombinant Mouse Common beta Chain Protein

SDS-Page: Recombinant Mouse Common beta Chain Protein [NBC1-18483] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain. Molecular Weight: 14 kDa (125 aa), confirmed by MALDI-TOF.Concentration: 1 mg/ml (determined by BCA assay).

Bioactivity: Recombinant Mouse Common beta Chain Protein [NBC1-18483] - Mouse GM-CSF stimulates cell proliferation of the MC/9 mouse mast cells. The ED50 range is less or equal 0.06 ng/ml.

Product Discontinued

Recombinant Mouse Common beta Chain Protein Summary

• Description: An un-tagged recombinant protein corresponding to the amino acids 18-141 of Mouse Common beta Chain
  
  

  Source: E.coli

  Amino Acid Sequence: MAPTRSPITV TRPWKHVEAI KEALNLLDDM PVTLNEEVEV VSNEFSFKKL TCVQTRLKIF EQGLRGNFTK LKGALNMTAS YYQTYCPPTP ETDCETQVTT YADFIDSLKT FLTDIPFECK KPVQK

• Details of Functionality: Mouse GM-CSF stimulates cell proliferation of the MC/9 mouse mast cells. The ED50 range is less or equal 0.06 ng/ml.
• Source: E. coli
• Protein/Peptide Type: Biologically Active Protein
• Gene: CSF2RB
• Purity: >95%, by SDS-PAGE
• Endotoxin Note: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 14 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Mouse Common beta Chain Protein

• beta, low-affinity
• CD131 antigen
• CD131
• CDw131
• colony stimulating factor 2 receptor, beta, low-affinity(granulocyte-macrophage)
• Common beta Chain
• CSF2RB
• cytokine receptor common subunit beta
• GM-CSF R beta
• GM-CSF/IL-3/IL-5 receptor common beta subunit
• GM-CSF/IL-3/IL-5 receptor common beta-chain
• IL-3 R beta
• IL3RB
• IL-5 R beta
• interleukin 3 receptor/granulocyte-macrophage colony stimulating factor 3receptor, beta (high affinity)

Background

GM-CSF is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It is produced in by endothelial cells, monocytes, fibroblasts and T-lymphocytes. GM-CSF inhibits neutrophil migration and enhances the functional activity of the mature end-cells. Mouse GM-CSF protein is 125 amino acids and shares approximately 60% homology with the human GM-CSF protein. Recombinant mouse GM-CSF was expressed in E.coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CSF2RB