Common beta Chain Antibody

Western Blot: Common beta Chain Antibody [NBP1-31771] - Sample (30 ug of whole cell lysate) A: H1299 7. 5% SDS PAGE; antibody diluted at 1:1000.

Immunohistochemistry-Paraffin: Common beta Chain Antibody [NBP1-31771] - Paraffin-embedded OVCAR3 xenograft, using antibody at 1:100 dilution.

Product Discontinued

Common beta Chain Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human Common beta Chain. The exact sequence is proprietary.
• Localization: Membrane, Single-pass type I membrane protein
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CSF2RB
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 97 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Common beta Chain Antibody

• beta, low-affinity
• CD131 antigen
• CD131
• CDw131
• colony stimulating factor 2 receptor, beta, low-affinity(granulocyte-macrophage)
• Common beta Chain
• CSF2RB
• cytokine receptor common subunit beta
• GM-CSF R beta
• GM-CSF/IL-3/IL-5 receptor common beta subunit
• GM-CSF/IL-3/IL-5 receptor common beta-chain
• IL-3 R beta
• IL3RB
• IL-5 R beta
• interleukin 3 receptor/granulocyte-macrophage colony stimulating factor 3receptor, beta (high affinity)

Background

Interleukin-3 receptor (IL-3R) is a heterodimer cytokine composed of alpha chain and beta chain. IL-3R beta subunit (CD131, beta C) is a common shared beta chain of the receptor for granulocyte- macrophage colony-stimulating factor (GM-CSF) and IL-5 (1). While the beta chain can not bind to the ligand alone, but it is essential for high affinity ligand binding (2). The cytoplasmic portion of the beta chain contains the major domains necessary for ligand-induced proliferation (3). IL-3R beta is expressed in neutrophil, eosinophil, monocyte, and hematpoietic stem cells. A point mutation leading to defective IL-3R beta expression has been associated to Human pulmonary alveolar proteinosis (PAP), a rare cause of respiratory failure (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: CSF2RB