Collagen XXV alpha 1 Antibody

Images

 
Western Blot: Collagen XXV alpha 1 Antibody [NB300-289] - Stable HEK293 cell line expressing CLAC-P using NB300-289. Lane 1: full-length CLAC-P (lysate). Lane 2: secreted form of CLAC-P (media). Lane 3: COLXIII ...read more
Immunohistochemistry: Collagen XXV Antibody [NB300-289] - Immunostain of the neocortex of Alzheimer's diseased brain sections (floating, fixed with 10% formalin for 24 hrs) using NB 300-289. At high magnification, NB ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Collagen XXV alpha 1 Antibody Summary

Immunogen
A synthetic peptide mapping to residues 430-443 [DYNGNLHEALQRIT] of the NC3 region of human and mouse CLAC-P.
Specificity
This detects full-length CLAC-P and the secreted form of CLAC-P protein. It does not react with full-length or secreted forms of COLXIII protein or the COLXXIII protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
COL25A1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
This antibody has been tested in Western blot and immunohistochemistry-Paraffin. Suggested Starting Dilutions:* Western Blot: 0.2 ug/ml (ECL Overexpressed protein)

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Collagen XXV alpha 1 Lysate (NBL1-09352)

Reactivity Notes

This antibody works in human and is expected to react with mouse protein.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Collagen XXV alpha 1 Antibody

  • Alzheimer disease amyloid-associated protein
  • AMY
  • CLAC
  • CLACP
  • CLAC-P
  • COL25A1
  • Collagen 25
  • collagen alpha-1(XXV) chain
  • Collagen XXV alpha 1
  • collagen, type XXV, alpha 1
  • Collagen-25
  • collagen-like Alzheimer amyloid plaque component
  • collagenous Alzheimer amyloid plaque component

Background

Antibodies against senile plaque (SP) amyloid obtained a clone 9D2, which labeled amyloid fibrils in SPs and reacted with approximately 50/100 kDa polypeptides in Alzheimer's disease (AD) brains. The 9D2 antigen was identified as CLAC (collagen-like Alzheimer amyloid plaque component), and its precursor as CLAC-P/collagen type XXV. Both secreted and membrane-tethered forms of CLAC-P/collagen type XXV specifically bound to fibrillized Abeta, implicating these proteins in beta-amyloidogenesis and neuronal degeneration in AD.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol COL25A1
Entrez