Human Collagen XVII ELISA Kit (Colorimetric)

ELISA: Human Collagen XVII ELISA Kit (Colorimetric) [NBP2-75837] - Samples were spiked with high concentrations of Human Collagen XVII and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human Collagen XVII ELISA Kit (Colorimetric) [NBP2-75837] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 3.13 - 200 ng/mL
• Sensitivity: 1.88 ng/mL

Human Collagen XVII ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human COL17 in samples. No significant cross-reactivity or interference between Human COL17 and analogues was observed.
• Standard Curve Range: 3.13 - 200 ng/mL
• Sensitivity: 1.88 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5.57%
• Intra-Assay: CV% < 5.48%
• Spike Recovery: 91-110%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: COL17A1

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Collagen XVII ELISA Kit (Colorimetric)

• 180 kDa bullous pemphigoid antigen 2
• BA16H23.2
• BP180
• BP180alpha 1 type XVII collagen
• BPA-2
• BPAG2
• BPAG2bA16H23.2 (collagen, type XVII, alpha 1 (BP180))
• bullous pemphigoid antigen 2 (180kD)
• Bullous pemphigoid antigen 2
• COL17A1
• Collagen 17
• collagen alpha-1(XVII) chain
• Collagen XVII
• collagen XVII, alpha-1 polypeptide
• collagen, type XVII, alpha 1
• Collagen-17
• ERED
• FLJ60881
• KIAA0204
• LAD-1

Background

Collagen XVII encodes the alpha chain of type XVII collagen. Unlike most collagens, collagen XVII is a transmembrane protein. Collagen XVII is a structural component of hemidesmosomes, multiprotein complexes at the dermal-epidermal basement membrane zone that mediate adhesion of keratinocytes to the underlying membrane. Mutations in this gene are associated with both generalized atrophic benign and junctional epidermolysis bullosa. Two homotrimeric forms of type XVII collagen exist. The full length form is the transmembrane protein. A soluble form, referred to as either ectodomain or LAD-1, is generated by proteolytic processing of the full length form. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Collagen XVII ELISA Kit (NBP2-75837). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: COL17A1