Collagen XVII Antibody (NC16a-3)

Product Discontinued

Collagen XVII Antibody (NC16a-3) Summary

• Immunogen: Immunogen: purified recombinant NC16a-domain of collagen XVII. The epitope has been mapped to aa545-557 (PMID: 19158842).
• Specificity: Does not cross-react with mouse and rat skin
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: COL17A1
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:2 - 1:40
  • Western Blot 1:100 - 1:1000

Packaging, Storage & Formulations

• Storage: Lyophilized antibody can be kept at 4C for up to 3 months and should be kept at -20C for long-term storage. To avoid freeze-thaw cycles, reconstituted antibody should be aliquoted before freezing for short-term storage (-20C) or for long-term storage (-80C). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
• Buffer: PBS, pH 7.4
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A or G purified from hybridoma culture supernatant
• Reconstitution Instructions: Reconstitute with 0.25 ml water.

Alternate Names for Collagen XVII Antibody (NC16a-3)

• 180 kDa bullous pemphigoid antigen 2
• BA16H23.2
• BP180
• BP180alpha 1 type XVII collagen
• BPA-2
• BPAG2
• BPAG2bA16H23.2 (collagen, type XVII, alpha 1 (BP180))
• bullous pemphigoid antigen 2 (180kD)
• Bullous pemphigoid antigen 2
• COL17A1
• Collagen 17
• collagen alpha-1(XVII) chain
• Collagen XVII
• collagen XVII, alpha-1 polypeptide
• collagen, type XVII, alpha 1
• Collagen-17
• ERED
• FLJ60881
• KIAA0204
• LAD-1

Background

Collagen XVII encodes the alpha chain of type XVII collagen. Unlike most collagens, collagen XVII is a transmembrane protein. Collagen XVII is a structural component of hemidesmosomes, multiprotein complexes at the dermal-epidermal basement membrane zone that mediate adhesion of keratinocytes to the underlying membrane. Mutations in this gene are associated with both generalized atrophic benign and junctional epidermolysis bullosa. Two homotrimeric forms of type XVII collagen exist. The full length form is the transmembrane protein. A soluble form, referred to as either ectodomain or LAD-1, is generated by proteolytic processing of the full length form. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: COL17A1
• Uniprot:
  • Q9UMD9(Human)