Collagen XIII alpha 1 Antibody (584718)

Collagen XIII a1 was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human Collagen XIII a1 Monoclonal Antibody (Catalog # MAB6346) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to basement membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: IHC
• Clone: 584718
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Collagen XIII alpha 1 Antibody (584718) Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Collagen XIII alpha 1
  Glu109-Pro668
  Accession # Q5TAT6
• Specificity: Detects human Collagen XIII alpha 1 in direct ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant mouse Collagen XIII alpha 1 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: COL13A1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Collagen XIII alpha 1 Antibody (584718)

• COL13A1
• collagen alpha-1(XIII) chain
• Collagen XIII alpha 1
• collagen, type XIII, alpha 1
• FLJ42485

Background

COL13A1 (collagen 13-alpha 1) is a 95 kDa member of the transmembrane group of the collagen family of proteins. It is a type II transmembrane glycoprotein that is expressed by multiple cell types, including fibroblasts, endothelial cells, and cardiac muscle. COL13A1 forms disulfide-linked homotrimers and participates in cell adhesion by binding to the Integrin alpha 1 subunit, Nidogen-2, and Fibronectin. Human COL13A1 is 717 amino acids (aa) in length. It has an N‑terminal 44 aa cytoplasmic region plus a 656 aa extracellular domain (ECD) (aa 62-717). The ECD contains four non-collagenous (NC) regions (aa 1‑121, aa 217-269, aa 442-463, and aa 700-717) interspersed among three collagenous (COL) domains (aa 122-216, aa 270-441, and aa 464-699). Multiple splice forms exist and typically involve deletions of 12-30 aa between aa 220-705. Proteolytic cleavage after Arg108 generates an 85-90 kDa soluble form. Over aa 109-668 (based on a human isoform [NP_542992} that shows a deletion of aa 239-260, aa 551-565, and aa 616-627), human COL13A1 shares 91% aa sequence identity with mouse COL13A1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: COL13A1
• Uniprot:
  • Q5TAT6(Human)