Human Collagen VII ELISA Kit (Chemiluminescence)

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ELISA: Human Collagen VII ELISA Kit (Chemiluminescence) [NBP2-75881] - Samples were spiked with high concentrations of Human Collagen VII and diluted with Reference Standard & Sample Diluent to produce samples with ...read more
ELISA: Human Collagen VII ELISA Kit (Chemiluminescence) [NBP2-75881] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
31.25-2000 pg/mL
Sensitivity
18.75 pg/mL

Order Details

Human Collagen VII ELISA Kit (Chemiluminescence) Summary

Specificity
This kit recognizes Human COL7 in samples. No significant cross-reactivity or interference between Human COL7 and analogues was observed.
Standard Curve Range
31.25-2000 pg/mL
Sensitivity
18.75 pg/mL
Assay Type
Sandwich-CLIA
Inter-Assay
CV% < 9.63%
Intra-Assay
CV% < 9.2%
Spike Recovery
87-114%
Sample Volume
100 uL
Kit Type
ELISA Kit (Chemiluminescence)
Gene
COL7A1

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro CLIA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Substrate Reagent A
  12. Substrate Reagent B

Alternate Names for Human Collagen VII ELISA Kit (Chemiluminescence)

  • Collagen 7
  • collagen alpha-1(VII) chain
  • collagen VII, alpha-1 polypeptide
  • collagen, type VII, alpha 1
  • Collagen-7
  • EBD1
  • EBDCT
  • EBR1
  • epidermolysis bullosa, dystrophic, dominant and recessive
  • LC collagen
  • long chain collagen
  • Long-chain collagen

Background

Type VII collagen fibrils are composed of three identical alpha collagen chains. It's location is restricted to the basement zone beneath stratified squamous epithelia. It functions as an anchoring fibril between the external epithelia and the underlying stroma. Mutations in the collagen V11 gene are associated with all forms of dystrophic epidermolysis bullosa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol COL7A1