Collagen IV Antibody

Immunohistochemistry-Paraffin: Collagen IV Antibody [NBP1-26549] - Human gastric cancer tissue was stained with Goat IgG-UNLB isotype control (left) and Goat Anti-Type IV Collagen-UNLB; (right) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP DAB, and hematoxylin.

• Reactivity: Hu, Mu, Rt, Bv, Pm
• Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.4 mg/ml

Collagen IV Antibody Summary

• Immunogen: Goats were hyperimmunized with human type IV collagen.
• Specificity: Reacts with conformational determinants on human type IV collagen as demonstrated by ELISA. May react with type IV collagen from other species. Exhibits <10% cross reactivity with collagen type I, II, III, V and VI. The antibody has not been tested for reactivity with other ECM proteins (e.g., laminin, fibronectin).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: COL4A1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:100-1:2000
  • ELISA 1:1000-1:4000
  • Immunocytochemistry/Immunofluorescence 1:10-1:20
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:20-1:40
  • Immunohistochemistry-Paraffin 1:10-1:500
• Control:

  Collagen IV alpha 1 Knockout HeLa Cell Lysate

Reactivity Notes

Monkey reactivity reported in scientific literature (PMID: 27417518)

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 0.1M BBS (pH 8.2)
• Preservative: No Preservative
• Concentration: 0.4 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Collagen IV Antibody

• Collagen IV

Background

Collagen IV is a major constituent of the basement membranes along with laminins, proteoglycans and enactins. It is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. It can form insoluble fibers with high tensile strength. Collagen IV is useful in detecting the loss of parts of basement membranes in carcinomas. This antibody is well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Collagen IV Antibody (NBP1-26549)(4)

Publications using NBP1-26549

• Chang YC, Hahn RA, Gordon MK et al. A type IV collagenous inhibitor, N-hydroxy-3-phenyl-2-(4-phenylbenzenesulfonamido) propanamide (BiPS), suppresses skin injury induced by sulfur mustard Toxicol. Appl. Pharmacol. May 29 2020 [PMID: 32479919] (ICC/IF, Mouse)
• Koundal S, Elkin R, Nadeem S et al. Optimal Mass Transport with Lagrangian Workflow Reveals Advective and Diffusion Driven Solute Transport in the Glymphatic System Sci Rep Feb 6 2020 [PMID: 32029859] (IHC-Fr, IHC, Rat)
• Gorman DE, Wu T, Gilpin SE, Ott HC. A Fully Automated High-Throughput Bioreactor System for Lung Regeneration. Tissue Eng Part C Methods. Oct 26 2018 [PMID: 30362896] (IHC-P, Rat)
• Slayden OD, Lee DO, Yao S, Jensen JT. Polidocanol induced tubal occlusion in nonhuman primates: immunohistochemical detection of collagen I-V. Contraception. Nov 1 2016 [PMID: 27417518] (IHC, Monkey)

Bioinformatics

• Gene Symbol: COL4A1