Collagen IV Antibody

Immunohistochemistry-Frozen: Collagen IV Antibody [NBP1-26549] - Left panel: NBP1-26549 signal, identifying Collagen IV in extracellular basement membrane of brain endothelial cells. Right panel: counterstained image with DAPI. Mouse brain tissue (12um thickness) was incubated overnight with NBP1-26549 (1:400) in PBS 1x at 4C. The day after, the slide was washed 3 x PBS 1x and then incubated with secondary anti-goat antibody (Alex Fluor 568-conjugated), 1:1000 for 2 hours at r.t. After counterstaining with DAPI, image was acquired with confocal microscopy. Left panel: NBP1-26549 signal, identifying Collagen IV in extracellular basement membrane of brain endothelial cells. Right panel: counterstained image with DAPI. Image from verified customer review.

Immunohistochemistry-Paraffin: Collagen IV Antibody [NBP1-26549] - Human gastric cancer tissue was stained with Goat IgG-UNLB isotype control (left) and Goat Anti-Type IV Collagen-UNLB; (right) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP DAB, and hematoxylin.

Immunohistochemistry: Collagen IV Antibody [NBP1-26549] - Col-IV in the rhesus macaque and baboon intramural tube. Images of open fallopian tube (m, o) were obtained from control animals. Occluded tube (n, p) were from animals treated with 5% PF. Arrows indicate fibrotic response. All images were captured at original magnification 200x. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27417518) licensed under a CC-BY licence.

Immunohistochemistry: Collagen IV Antibody [NBP1-26549] - Photographs showing Col-IV staining in rhesus macaque ampulla. Staining for Col-IV was undetectable from the epithelium. No increase in collagen was detected in the PF-treated animals. Control inset shows staining with an irrelevant antibody. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27417518) licensed under a CC-BY licence.

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.4 mg/ml

Collagen IV Antibody Summary

• Immunogen: Goats were hyperimmunized with human type IV collagen.
• Specificity: Reacts with conformational determinants on human type IV collagen as demonstrated by ELISA. May react with type IV collagen from other species. Exhibits <10% cross reactivity with collagen type I, II, III, V and VI. The antibody has not been tested for reactivity with other ECM proteins (e.g., laminin, fibronectin).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: COL4A1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:1000-1:4000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: IHC, IHC-FR reported in (PMID: 32029859), ICC/IF reported in (PMID: 32479919), IHC-P reported in (PMID: 27417518).

Reactivity Notes

Monkey reactivity reported in scientific literature (PMID: 27417518), Mouse reactivity reported in scientific literature (PMID: 32479919), Rat reactivity reported in scientific literature (PMID: 30362896).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 0.1M BBS (pH 8.2)
• Preservative: No Preservative
• Concentration: 0.4 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Collagen IV Antibody

• Collagen IV

Background

Collagen IV is a major constituent of the basement membranes along with laminins, proteoglycans and enactins. It is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. It can form insoluble fibers with high tensile strength. Collagen IV is useful in detecting the loss of parts of basement membranes in carcinomas. This antibody is well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Collagen IV Antibody (NBP1-26549)(11)

Publications using NBP1-26549

• Juin SK, Pushpakumar S, Sen U. GYY4137 Regulates Extracellular Matrix Turnover in the Diabetic Kidney by Modulating Retinoid X Receptor Signaling Biomolecules Oct 7 2021 [PMID: 34680110]
• Irizarry B, Davis J, Zhu X et al. Human cerebral vascular amyloid contains both anti-parallel and parallel in-register A Beta 40 fibrils Journal of Biological Chemistry Sep 1 2021 [PMID: 34599967] (ICC/IF, Human)
• Swindell WR, Randhawa M, Quijas G et al. Tetrahexyldecyl Ascorbate (THDC) Degrades Rapidly under Oxidative Stress but Can Be Stabilized by Acetyl Zingerone to Enhance Collagen Production and Antioxidant Effects Int J Mol Sci Aug 15 2021 [PMID: 34445461] (Human, ELISA)
  
  Details:
  Citation using the Biotin format of this antibody.
• Dudek M, Pfister D, Donakonda S et al. Auto-aggressive CXCR6+ CD8 T cells cause liver immune pathology in NASH Nature Mar 24 2021 [PMID: 33762736]
• Pushpakumar S, Kundu S, Weber G, Sen U Exogenous hydrogen sulfide and miR-21 antagonism attenuates macrophage-mediated inflammation in ischemia reperfusion injury of the aged kidney GeroScience Jan 12 2021 [PMID: 33433751]
• Kuang, X, Sun, L Et al. A novel missense mutation of COL4A5 gene alter collagen IV alpha 5 chain to cause X-linked Alport syndrome in a Chinese family. Transl Pediatr Oct 1 2020 [PMID: 33209720] (IHC, Rat)
• Coate KC, Cha J, Shrestha S et al. SARS-CoV-2 Cell Entry Factors ACE2 and TMPRSS2 Are Expressed in the Microvasculature and Ducts of Human Pancreas but Are Not Enriched in beta Cells Int J Mol Sci Nov 12 2020 [PMID: 33207245]
• Chang YC, Hahn RA, Gordon MK et al. A type IV collagenous inhibitor, N-hydroxy-3-phenyl-2-(4-phenylbenzenesulfonamido) propanamide (BiPS), suppresses skin injury induced by sulfur mustard Toxicol. Appl. Pharmacol. May 29 2020 [PMID: 32479919] (ICC/IF, Mouse)
• Koundal S, Elkin R, Nadeem S et al. Optimal Mass Transport with Lagrangian Workflow Reveals Advective and Diffusion Driven Solute Transport in the Glymphatic System Sci Rep Feb 6 2020 [PMID: 32029859] (IHC-Fr, IHC, Rat)
• Gorman DE, Wu T, Gilpin SE, Ott HC. A Fully Automated High-Throughput Bioreactor System for Lung Regeneration. Tissue Eng Part C Methods. Oct 26 2018 [PMID: 30362896] (IHC-P, Rat)
• Slayden OD, Lee DO, Yao S, Jensen JT. Polidocanol induced tubal occlusion in nonhuman primates: immunohistochemical detection of collagen I-V. Contraception. Nov 1 2016 [PMID: 27417518] (IHC, Monkey)

Review for Collagen IV Antibody (NBP1-26549) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: STEFANO Benvegnu IHC-Fr Mouse 05/24/2022

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Adult mouse brain tissue
• Species: Mouse
• Lot: H2915-QI22

Comments

• Comments: Mouse brain tissue &#40;12um thickness&#41; was incubated overnight with NBP1-26549 &#40;1:400&#41; in PBS 1x at 4C. The day after, the slide was washed 3 x PBS 1x and then incubated with secondary anti-goat antibody &#40;Alex Fluor 568-conjugated&#41;, 1:1000 for 2 hours at r.t. After counterstaining with DAPI, image was acquired with confocal microscopy. Left panel: NBP1-26549 signal, identifying Collagen IV in extracellular basement membrane of brain endothelial cells. Right panel: counterstained image with DAPI.

Bioinformatics

• Gene Symbol: COL4A1