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Human Collagen IV alpha 1 ELISA Kit (Chemiluminescence)

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Human Collagen IV alpha 1 ELISA Kit (Chemiluminescence) - Standard Curve Reference
Human Collagen IV alpha 1 ELISA Kit (Chemiluminescence) - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
62.50-4000 pg/mL
Sensitivity
37.50 pg/mL

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Human Collagen IV alpha 1 ELISA Kit (Chemiluminescence) Summary

Specificity
This kit recognizes Human COL4a1 in samples. No significant cross-reactivity or interference between Human COL4a1 and analogues was observed.
Standard Curve Range
62.50-4000 pg/mL
Sensitivity
37.50 pg/mL
Assay Type
Sandwich-CLIA
Inter-Assay
CV% < 9.22%
Intra-Assay
CV% < 9.4%
Spike Recovery
83-117%
Sample Volume
100 uL
Kit Type
ELISA Kit (Chemiluminescence)
Gene
COL4A1

Packaging, Storage & Formulations

Storage
Storage is content dependent.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100×)
  3. Certificate of Analysis
  4. Concentrated HRP Conjugate (100×)
  5. Concentrated Wash Buffer (25×)
  6. Plate Sealer
  7. Product Description
  8. Reference Standard
  9. Micro CLIA Plate(Dismountable)
  10. HRP Conjugate Diluent
  11. Reference Standard & Sample Diluent
  12. Substrate Reagent A
  13. Substrate Reagent B

Alternate Names for Human Collagen IV alpha 1 ELISA Kit (Chemiluminescence)

  • alpha-1 chain
  • Arresten
  • COL4A1 NC1 domain
  • COL4A1
  • collagen alpha-1(IV) chain
  • Collagen IV alpha 1
  • collagen IV, alpha-1 polypeptide
  • collagen, type IV, alpha 1
  • Collagen-4
  • EC 3.4.23
  • EC 6.3.1.2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Immunohistochemistry-Paraffin: Collagen I Antibody [NB600-408] - Rat colon tissue stained with Collagen I antibody (red) and Hoechst (blue). IHC-P image submitted by a verified customer review.
Immunocytochemistry/Immunofluorescence: Collagen I Antibody [NB600-408] - Myofibroblast differentiation requires CD271/NGF activation. Representative alphaSMA (red) and COLLAGEN 1 (red) immunostaining with DAPI (blue) in non-treated (control), TGFbeta-treated cells with or without gamma-secretase inhibitor. Scale bars: 50 um Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-019-09992-3) licensed under a CC-BY licence.
Western Blot: Collagen I Antibody [NB600-408] - Lane 1: Human Collagen Type 1. Lane 2: None. Load: 50 ng per lane. Primary antibody: Collagen Type I antibody at 1:1000 overnight at 4C. Secondary antibody: DyLight 649 rabbit secondary antibody at 1:20000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 139 &amp; 130 kDa, 139 &amp; 130 kDa for Collagen Type I. Other Band(s): Collagen Type I splice variants and isoforms.
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Immunocytochemistry/Immunofluorescence: Fibronectin Antibody [NBP1-91258] - NIH-3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-Fibronectin at 2 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at 1:500. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at 1:1000 and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
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Immunohistochemistry-Paraffin: COL1A2 Antibody [NBP2-92790] - Paraffin-embedded rat brain using COL1A2.
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Western Blot: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of control (C; open bar; n = 6) and hypothyroid (Hypo; solid bar; n = 6) rabbits. Representative immunoblot showing the expression of aromatase. Image collected and cropped by CiteAb from the following publication (https://www.hindawi.com/journals/bmri/2017/3795950/), licensed under a CC-BY licence.
Immunocytochemistry/Immunofluorescence: Aromatase Antibody [NB100-1596] - U2OS cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Aromatase Antibody NB100-1596 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Immunohistochemistry-Paraffin: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of control rabbits. Immunoreactivity of antiaromatase (+) was identified in granulosa cells of antral follicles (tertiary, tf; Graafian, gf) of control ovaries. Nonlabeling was observed when the primary antibody was omitted. Bars: 200 um (1st image) and 20 um (2nd image). Granulose cells, gc; theca cells, tc. Image collected and cropped by CiteAb from the following publication (https://www.hindawi.com/journals/bmri/2017/3795950/), licensed under a CC-BY licence.
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  Human Collagen IV alpha 1 ELISA Kit (Chemiluminescence) Manual
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Bioinformatics

Gene Symbol COL4A1