Collagen IV alpha 1 Antibody

Product Discontinued

Collagen IV alpha 1 Antibody Summary

• Immunogen: A synthetic peptide made to an internal portion of the human Collagen IV protein (between residues 100-200) [UniProt P02462]
• Clonality: Polyclonal
• Host: Rabbit
• Gene: COL4A1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Application Notes: Western blot (WB) analysis of Collagen IV (G46) pAb in extracts from HeLa cell. Immunohistochemistry (IHC) analyzes of Collagen IV (G46) pAb in paraffin-embedded human breast carcinoma tissue. Reacts with Rat as reported in the literature (PMID 22413961)Immunocytochemistry/Immunofluorescence was reported in scientific literature.
  
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 160 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human and Mouse, Rat

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Collagen IV alpha 1 Antibody

• alpha-1 chain
• Arresten
• COL4A1 NC1 domain
• COL4A1
• collagen alpha-1(IV) chain
• Collagen IV alpha 1
• collagen IV, alpha-1 polypeptide
• collagen, type IV, alpha 1
• Collagen-4
• EC 3.4.23
• EC 6.3.1.2

Background

Collagen IV is a major constituent of the basement membranes along with laminins, proteoglycans and enactins. It is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. It can form insoluble fibers with high tensile strength. Collagen IV is useful in detecting the loss of parts of basement membranes in carcinomas. This antibody is well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Collagen IV alpha 1 Antibody (NB100-91716)(6)

Publications using NB100-91716

• Liu G, Wang B, Li S et al. Human breast cancer decellularized scaffolds promote epithelial-to-mesenchymal transitions and stemness of breast cancer cells in vitro J. Cell. Physiol. 2018-11-27 [PMID: 30478896] (ICC/IF, Human)
• Chen SY, Lin JS, Lin HC et al. Dependence of fibroblast infiltration in tumor stroma on type IV collagen-initiated integrin signal through induction of platelet-derived growth factor Biochim Biophys Acta. 2015-05-01 [PMID: 25686533]
• Chen SY, Lin JS, Yang BC. Modulation of tumor cell stiffness and migration by type IV collagen through direct activation of integrin signaling pathway. Arch. Biochem. Biophys. 2014-05-10 [PMID: 24823860] (WB, Mouse, Human)
• Lekawanvijit S, Kumfu S, Wang BH et al. The Uremic Toxin Adsorbent AST-120 Abrogates Cardiorenal Injury Following Myocardial Infarction. PLoS One 2013-12-13 [PMID: 24349545] (WB, Rat)
• Liu, J, Takase, I, Hakucho, A et al. Carvedilol Attenuates the Progression of Alcohol Fatty Liver Disease in Rats Alcoholism: Clinical and Experimental Research 2012-01-01 [PMID: 22413959] (WB, Rat)
• Surlin P, Rauten AM, Pirici D et al. Collagen IV and MMP-9 expression in hypertrophic gingiva during orthodontic treatment Rom J Morphol Embryol 2012-01-01 [PMID: 22395516] (IF/IHC, ICC/IF, Human)

Bioinformatics

• Gene Symbol: COL4A1
• Entrez:
  • 12826(Mouse)