Collagen IV alpha 1 Antibody (1106301) [Alexa Fluor® 647]

• Reactivity: Hu
• Applications: IHC
• Clone: 1106301
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 647

Collagen IV alpha 1 Antibody (1106301) [Alexa Fluor® 647] Summary

• Immunogen: Synthetic peptide
  Accession # P02462
• Specificity: Detects recombinant human COL4A1 protein in Direct ELISA.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Collagen IV alpha 1 Antibody (1106301) [Alexa Fluor® 647]

• alpha-1 chain
• Arresten
• COL4A1 NC1 domain
• COL4A1
• collagen alpha-1(IV) chain
• Collagen IV alpha 1
• collagen IV, alpha-1 polypeptide
• collagen, type IV, alpha 1
• Collagen-4
• EC 3.4.23
• EC 6.3.1.2

Background

COL4A1 (collagen IV alpha 1) is a 185 kDa member of the type IV collagen family.  It is a secreted glycoprotein that is expressed by multiple cell types, including fibroblasts, keratinocytes and endothelial cells.  Two COL4A1 molecules interact with a 170 kDa alpha 2 chain to form a collagen IV triple helix.  This helix further interacts with other helices to generate covalent oligomers that form a scaffold in the basement membrane.  Mature human COL4A1 is 1642 amino acids (aa) in length.  It has an N-terminal “7S” proregion (aa 28-172), a central collagenous domain that contains multiple Gly-based repeats (aa 173-1440), and a C-terminal domain that is proteolytically cleaved to generate a 25-28 kDa NC1 globular segment that has potent antiangiogenic activity (aa 1441-1669).  Multiple splice forms exist.  One shows a deletion of aa 499-849, a second shows a seven aa substitution for aa 513-1669, and a third shows a seven aa substitution for aa 958-1669.  Over aa 1441-1669, human COL4A1 is 97% aa identical to mouse COL4A1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P02462(Human)