COG7 Antibody - BSA Free

Western Blot: COG7 Antibody [NBP2-14914] - Sample (20 ug of whole cell lysate) A: HeLa membrane 7. 5% SDS PAGE; antibody diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: COG7 Antibody [NBP2-14914] - Analysis of methanol-fixed HeLa, using COG7 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

COG7 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit COG7 Antibody - BSA Free (NBP2-14914) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human COG7. The exact sequence is proprietary.
• Predicted Species: Mouse (97%), Rat (96%), Bovine (97%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: COG7
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 86 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Zebrafish (81%), Xenopus laevis (84%), Chicken (82%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for COG7 Antibody - BSA Free

• CDG2E
• component of oligomeric golgi complex 7COG complex subunit 7
• conserved oligomeric Golgi complex subunit 7

Background

Multiprotein complexes are key determinants of Golgi apparatus structure and its capacity for intracellular transport and glycoprotein modification. Several complexes have been identified, including the Golgi transport complex (GTC), the LDLC complex, which is involved in glycosylation reactions, and the SEC34 complex, which is involved in vesicular transport. These 3 complexes are identical and have been termed the conserved oligomeric Golgi (COG) complex, which includes COG7 (Ungar et al., 2002 [PubMed 11980916]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: COG7
• Uniprot:
  • P83436(Human)