Recombinant Human Cofilin 2 Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE

Order Details

Recombinant Human Cofilin 2 Protein Summary

Description
A recombinant protein with a His-tag corresponding to amino acids 1 to 166 of Human Cofilin 2

Source: E. coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MASGVTVNDE VIKVFNDMKV RKSSTQEEIK KRKKAVLFCL SDDKRQIIVE EAKQILVGDI GDTVEDPYTS FVKLLPLNDC RYALYDATYE TKESKKEDLV FIFWAPESAP LKSKMIYASS KDAIKKKFTG IKHEWQVNGL DDIKDRSTLG EKLGGNVVVS LEGKPL
Preparation
Method
E.coli
Protein/Peptide Type
Recombinant Protein
Gene
CFL2
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Application Notes
The purity of this protein is > 90% by SDS-PAGE. Molecular weight is 20.9kDa (186a), confirmed by MALDI-TOF
Theoretical MW
20.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 10% glycerol, 50mM Nacl
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human Cofilin 2 Protein

  • cofilin 2 (muscle)
  • Cofilin, muscle isoform
  • cofilin-2
  • NEM7

Background

CFL2 protein belongs to the actin-binding proteins ADF family that contains 1 ADF-H domain. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. Defects in CFL2 are the cause of nemaline myopathy type 7 (NEM7). Recombinant human CFL2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol CFL2

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