Human Cofilin 2 ELISA Kit (Colorimetric)

ELISA: Human Cofilin 2 ELISA Kit (Colorimetric) [NBP2-75254] - Samples were spiked with high concentrations of Human Cofilin 2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human Cofilin 2 ELISA Kit (Colorimetric) [NBP2-75254] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 15.63 - 1000 pg/mL
• Sensitivity: 9.38 pg/mL

Human Cofilin 2 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human CFL2 in samples. No significant cross-reactivity or interference between Human CFL2 and analogues was observed.
• Standard Curve Range: 15.63 - 1000 pg/mL
• Sensitivity: 9.38 pg/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 4.79%
• Intra-Assay: CV% < 5.01%
• Spike Recovery: 85-106%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: CFL2

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Cofilin 2 ELISA Kit (Colorimetric)

• cofilin 2 (muscle)
• Cofilin, muscle isoform
• cofilin-2
• NEM7

Background

Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. (Gillett et al., 1996 [PubMed 8800436]). Cofilin-2 is a member of the AC group of proteins that also includes cofilin-1 (CFL1) and destrin (DSTN; MIM 609114), all of which regulate actin-filament dynamics (Bamburg et al., 1999 [PubMed 10461190]; Maciver and Hussey, 2002). The CFL2 gene encodes a skeletal muscle-specific isoform (Vartiainen et al., 2002 [PubMed 11809832]) localized to the thin filaments, where it exerts its effect on actin, in part through interactions with tropomyosins (Ono and Ono, 2002 [PubMed 11901171]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: CFL2