COCO/DAND5 Antibody (340531) [Alexa Fluor® 594]

• Reactivity: Mu
• Applications: WB
• Clone: 340531
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 594

COCO/DAND5 Antibody (340531) [Alexa Fluor® 594] Summary

• Immunogen: E. coli-derived recombinant mouse COCO
  Arg24-Leu185
  Accession # Q76LW6
• Specificity: Detects mouse COCO in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) COCO, rhDAN, recombinant mouse (rm) Gremlin, rmCerberus, and recombinant chicken Caronte is obse
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for COCO/DAND5 Antibody (340531) [Alexa Fluor® 594]

• CER2CRL2
• Cerberus 2
• Cerberus like-2
• cerberus-like 2
• Cerberus-like protein 2
• CERL2
• cerl-2
• CKTSF1B3
• CKTSF1B3MGC126849
• COCO
• Cysteine knot superfamily 1, BMP antagonist 3
• DAN domain family member 5
• DAN domain family, member 5
• DAND5
• Dante
• DTE
• FLJ38607
• GREM3
• GREM3cerberus 2
• gremlin-3
• SP1

Background

COCO, also known as DAND5, Dante, and CKTSF1B3, is a member of the DAN domain family of BMP antagonists that includes DAN (DAND1), Gremlin/Drm (DAND2), PRDC (Protein Related to Dan and Cerberus; DAND3), and Cerberus (DAND4). DAN family members contain a cysteine knot domain that is homologous to that found in other TGF-beta superfamily ligands such as BMPs that play important roles in tissue morphogenesis and developmental processes (1‑6). The mouse COCO cDNA encodes a 185 amino acid (aa) precursor with a 23 aa signal sequence (7, 8). COCO has eight Cys residues in the cysteine knot which places it in the CAN (or eight-membered ring) subfamily of BMP antagonists along with the other DAN family proteins (1). Mature mouse COCO shares 62% and 27% aa sequence identity with human and Xenopus COCO, respectively. It shares 22%‑27% aa sequence identity with mouse DAN, Gremlin, PRDC, and Cerberus. In Xenopus embryos, COCO is expressed by pluripotent ectodermal cells. Expression is abruptly downregulated prior to gastrulation, and the loss of ectodermal cell pluripotency is coincident with COCO downregulation (7). COCO is required for Xenopus left-right axis formation (9). It functions predominantly on the right side of the embryo, although it is equally expressed on both left and right sides (9). COCO binds and inhibits activin, BMP-4, GDF-3/derrière, Wnt8, and Xnr1 (7, 9). In mouse, COCO expression is elevated on the right side of Henson’s node at the early somite stage, in contrast to the left side expression of Nodal (8).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q76LW6()