COBRA1 Antibody

Images

 
Western Blot: COBRA1 Antibody [NBP1-05977] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NBP1-05977 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 ...read more
Immunohistochemistry-Paraffin: COBRA1 Antibody [NBP1-05977] - Human placenta. Antibody used at a dilution of 1:200 (1ug/ml).
Immunohistochemistry-Paraffin: COBRA1 Antibody [NBP1-05977] - human breast carcinoma. NBP1-05977 a dilution of 1:250.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
 

Independent Antibodies

       

Order Details

COBRA1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 530 and 580 of human cofactor of BRCA1 using the numbering given in entry NP_056271.2 (GeneID 25920).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
COBRA1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 2-5 ug/mg lysate
Theoretical MW
66 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for COBRA1 Antibody

  • cofactor of BRCA1DKFZp586B0519
  • KIAA1182negative elongation factor B
  • NELFB
  • NELF-Bnegative elongation factor protein B

Background

NELFB is a subunit of negative elongation factor (NELF), which also includes NELFA (WHSC2; MIM 606026), either NELFC or NELFD (TH1L; MIM 605297), and NELFE (RDBP; MIM 154040). NELF acts with DRB sensitivity-inducing factor (DSIF), a heterodimer of SPT4 (SUPT4H1; MIM 603555) and SPT5 (SUPT5H; MIM 602102), to cause transcriptional pausing of RNA polymerase II (see MIM 180660) (Narita et al., 2003 [PubMed 12612062]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for COBRA1 Antibody (NBP1-05977)

Discover related pathways, diseases and genes to COBRA1 Antibody (NBP1-05977). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on COBRA1.

COBRA1: A Key Player in Transcriptional Pausing
Co-factor of BRCA1, also known as COBRA1, was first identified as a protein that binds to the tumour suppressor protein encoded by the breast cancer susceptibility gene BRCA1 (1). It was subsequently found to be identical to subunit B of the Negative...  Read full blog post.

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Bioinformatics

Gene Symbol COBRA1
Entrez
Uniprot