COBRA1 Antibody (F7E4) - BSA Free

Western Blot: COBRA1 Antibody (F7E4) [NBP2-29529] - Western blot of neonatal rat brain lysate showing specific immunolabeling of the ~62K COBRA1 protein.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: F7E4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

COBRA1 Antibody (F7E4) - BSA Free Summary

• Description: Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage).
• Immunogen: Recombinant full length human COBRA1 purified from E. coli. Accession # Q8WX92
• Specificity: Specific for endogenous levels of the ~62 kDa COBRA1 protein.
• Preparation Method: Protein G purified culture supernatant
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: COBRA1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Theoretical MW: 62 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

The antibody has been directly tested for reactivity in Western blots with human, mouse and rat tissue. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Alternate Names for COBRA1 Antibody (F7E4) - BSA Free

• cofactor of BRCA1DKFZp586B0519
• KIAA1182negative elongation factor B
• NELFB
• NELF-Bnegative elongation factor protein B

Background

COBRA1 (cofactor of BRCA1) also known as NELF-B, is an essential component of the negative elongation factor (NELF) complex. COBRA1 has been shown to directly bind estrogen receptor alpha (ER-alpha) and repress ER-alpha-mediated transcription (Aiyar SE et al., 2004). COBRA1 has also been demonstrated to interact with c-Jun and c-Fos suggesting a role in modulation of the AP-1 pathway and therefore may be important in cell proliferation, differentiation, apoptosis and oncogeneis (Zhong H et al., 2004)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: COBRA1
• Entrez:
  • 25920(Human)
• Uniprot:
  • Q8WX92(Human)