Coagulation Factor VII Overexpression Lysate (Native)

Images

 
Western Blot: Factor VII Overexpression Lysate (Adult Normal) [NBL1-10412] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Factor VII.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Coagulation Factor VII Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC213143 and based on accession number NM_019616. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens coagulation factor VII (serum prothrombin conversion accelerator) (F7), transcript variant 2, mRNA.
Gene
F7

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Coagulation Factor VII

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Coagulation Factor VII Overexpression Lysate (Native)

  • coagulation factor VII (serum prothrombin conversion accelerator)
  • Coagulation Factor VII
  • EC 3.4.21
  • EC 3.4.21.21
  • Eptacog alfa
  • F7
  • FVII coagulation protein
  • proconvertin
  • Serum prothrombin conversion accelerator
  • SPCA

Background

This gene encodes coagulation factor VII which is a vitamin K-dependent factor essential for hemostasis. This factor circulates in the blood in a zymogen form, and is converted to an active form by either factor IXa, factor Xa, factor XIIa, or thrombin by minor proteolysis. Upon activation of the factor VII, a heavy chain containing a catalytic domain and a light chain containing 2 EGF-like domains are generated, and two chains are held together by a disulfide bond. In the presence of factor III and calcium ions, the activated factor then further activates the coagulation cascade by converting factor IX to factor IXa and/or factor X to factor Xa. Alternative splicing of this gene results in 2 transcripts. Defects in this gene can cause coagulopathy. [provided by RefSeq]. Transcript Variant: This variant (2) is the predominant form in normal liver. It does not include exon 1b and thus encodes a shorter signal peptide; the mature peptide products encoded by the two transcript variants are identical. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Coagulation Factor VII Lysate (NBL1-10412) (0)

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Bioinformatics

Gene Symbol F7
Entrez