Mouse Coagulation Factor VII ELISA Kit (Colorimetric) Summary
Standard Curve Range |
31.25-2000 pg/mL (example only; lot dependent) |
Sensitivity |
5.75 pg/mL |
Assay Type |
Sandwich ELISA |
Inter-Assay |
CV% < 8 |
Intra-Assay |
CV% < 3.1 |
Spike Recovery |
93-109% |
Sample Volume |
100 ul |
Kit Type |
ELISA Kit (Colorimetric) |
Gene |
F7 |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
|
- 96 well microplate coated with capture antibody
- Color Reagent A
- Color Reagent B
- Detection Antibody conjugated to HRP
- Dilution Buffer Concentration
- Sample Dilution Buffer Concentrate
- Standards
- Stop Solution
- Wash Buffer Concentrate
|
Alternate Names for Mouse Coagulation Factor VII ELISA Kit (Colorimetric)
Background
Factor VII is a 50kDa multi-domain, single chain plasma glycoprotein synthesized in the liver and having abnormal circulating concentration of between 400 and 600 ug/L. It is essential for blood coagulation. Factor VII is converted to the active, two chain, serine protease Factor VIIa through cleavage by thrombin, factor Xa, factor IXa or factor XIIa. Factor VIIa converts factor X to factor Xa, which in turn converts prothrombin to thrombin.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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