Human Coagulation Factor VII ELISA Kit (Chemiluminescence)

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ELISA: Human Coagulation Factor VII ELISA Kit (Chemiluminescence) [NBP2-67950] - Samples were spiked with high concentrations of Human Coagulation Factor VII and diluted with Reference Standard & Sample Diluent to ...read more
ELISA: Human Coagulation Factor VII ELISA Kit (Chemiluminescence) [NBP2-67950] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
15.63-1000 pg/mL
Sensitivity
9.38 pg/mL

Order Details

Human Coagulation Factor VII ELISA Kit (Chemiluminescence) Summary

Specificity
This kit recognizes Human F7 in samples. No significant cross-reactivity or interference between Human F7 and analogues was observed.
Standard Curve Range
15.63-1000 pg/mL
Sensitivity
9.38 pg/mL
Assay Type
Sandwich-CLIA
Inter-Assay
CV% < 9.52%
Intra-Assay
CV% < 7.87%
Spike Recovery
90-109%
Sample Volume
100 uL
Kit Type
ELISA Kit (Chemiluminescence)
Gene
F7

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro CLIA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Substrate Reagent A
  12. Substrate Reagent B

Alternate Names for Human Coagulation Factor VII ELISA Kit (Chemiluminescence)

  • coagulation factor VII (serum prothrombin conversion accelerator)
  • Coagulation Factor VII
  • EC 3.4.21
  • EC 3.4.21.21
  • Eptacog alfa
  • F7
  • FVII coagulation protein
  • proconvertin
  • Serum prothrombin conversion accelerator
  • SPCA

Background

Factor VII is a 50kDa multi-domain, single chain plasma glycoprotein synthesized in the liver and having abnormal circulating concentration of between 400 and 600 ug/L. It is essential for blood coagulation. Factor VII is converted to the active, two chain, serine protease Factor VIIa through cleavage by thrombin, factor Xa, factor IXa or factor XIIa. Factor VIIa converts factor X to factor Xa, which in turn converts prothrombin to thrombin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol F7