Coagulation Factor VII Antibody [Biotin]

Coagulation Factor VII was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Human Coagulation Factor VII Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2338) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (yellow; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

Coagulation Factor VII Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Coagulation Factor VII (R&D Systems, Catalog # 2338-SE)
  Ala39-Pro444
  Accession # NP_062562
• Specificity: Detects human Coagulation Factor VII in Western blots. In this format, approximately 10% cross-reactivity with recombinant mouse Coagulation Factor VII is observed and less than 5% cross-reactivity with recombinant human Factor XA is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: F7
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Coagulation Factor VII Antibody [Biotin]

• coagulation factor VII (serum prothrombin conversion accelerator)
• Coagulation Factor VII
• EC 3.4.21
• EC 3.4.21.21
• Eptacog alfa
• F7
• FVII coagulation protein
• proconvertin
• Serum prothrombin conversion accelerator
• SPCA

Background

Coagulation Factors VII and VIIa refer to the pro and active forms of the same protease, respectively (1). Factor VII is synthesized in the liver and circulates in the plasma where it binds to tissue factor (TF), an integral membrane protein found in a variety of cell types. Upon binding of TF, Factor VII is rapidly converted into VIIa. The resulting 1:1 complex of VIIa and TF initiates the coagulation pathway and has also important coagulation-independent functions such as angiognesis (2). The cleavage and activation of Coagulation Factors VII, IX, and X by VIIa:TF is phospholipid-dependent whereas the cleavage of small peptide substrates is not (1). The predominant splicing variant of Factor VII in normal liver corresponds to the 444 amino acid precursor (3, 4). After a signal peptide (residues 1 to 38), the mature chain can be further processed into the light chain (residues 39-190) and the heavy chain (residues 191-444).

1. Morrissey, J.H. (2004) in Handbook of Proteolytic Enzymes, Barrett, A.J. et al. eds. p. 1659. 
2. Versteeg, H.H. et al. (2003) Carcinogenesis 24:1009.
3. Hagen, F.S, et al. (1986) Proc. Natl. Acad. Sci. USA 83:2412.
4. O’Hara, P.J. et al. (1987) Proc. Natl. Acad. Sci. USA 84:5158.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: F7
• Uniprot:
  • NP_062562()