Recombinant Human Coagulation Factor II/Thrombin Protein

Product Discontinued

Recombinant Human Coagulation Factor II/Thrombin Protein Summary

• Description: An un-tagged denatured recombinant protein corresponding to the amino acids 328-622 of Human Prothrombin
  
  

  Source: E.coli

  Amino Acid Sequence: MTFGSGEADC GLRPLFEKKS LEDKTERELL ESYIDGRIVE GSDAEIGMSP WQVMLFRKSP QELLCGASLI SDRWVLTAAH CLLYPPWDKN FTENDLLVRI GKHSRTRYER NIEKISMLEK IYIHPRYNWR ENLDRDIALM KLKKPVAFSD YIHPVCLPDR ETAASLLQAG YKGRVTGWGN LKETWTANVG KGQPSVLQVV NLPIVERPVC KDSTRIRITD NMFCAGYKPD EGKRGDACEG DSGGPFVMKS PFNNRWYQMG IVSWGEGCDR DGKYGFYTHV FRLKKWIQKV IDQFGE

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: F2
• Purity: >80%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Application Notes: Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.
• Theoretical MW: 33.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 0.4M UREA, 10% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >80%, by SDS-PAGE

Alternate Names for Recombinant Human Coagulation Factor II/Thrombin Protein

• coagulation factor II (thrombin) receptor-like 2
• Coagulation factor II receptor-like 2 (protease-actovated receptor 3)
• Coagulation factor II receptor-like 2
• Coagulation Factor II
• F2
• PAR-3
• PAR3proteinase-activated receptor 3
• protease-activated receptor 3
• proteinase-activated receptor-3
• PT
• serine protease
• Thrombin receptor-like 2
• Thrombin

Background

Proteinase-activated receptor 3 (PAR3) is a member of the Proteinase-Activated Receptor subfamily. The enzyme thrombin is involved in the activation of platelets, leukocytes, endothelial cells, and mesenchymal cells at sites of vascular injury. These cellular responses are triggered through proteolytic activation of PAR3 and other receptors by thrombin. It is believed that PAR3 functions as a cofactor for the cleavage and activation of PAR4. PAR3 expression has been documented in blood, bone marrow, lung, spleen, and vessel. ESTs have been isolated from head/neck, skeletal muscle, and skin libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

FAQs for Prothrombin Recombinant Protein (NBP2-51676). (Showing 1 - 1 of 1 FAQ).

1. Do you have neutralizing antibodies for anti-mouse Thrombin?
   • We currently have 1 neutralizing Thrombin antibody (NBP1-95029), however it is an anti-human antibody and has only been validated in human samples. If you would like to test this antibody in mouse samples, you may be interested in participating in our Innovators Reward Program.

Bioinformatics

• Gene Symbol: F2