Porcine Coagulation Factor II/Thrombin ELISA Kit (Colorimetric)

ELISA: Coagulation Factor II/Thrombin ELISA Kit [Biotin] [KA1407] - The curve is provided for illustration only. A standard curve should be generated each time the assay is performed.

• Reactivity: Po
• Applications: ELISA, NULL
• Suitable Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum
• Standard Curve Range: 0.391 to 100 ng/mL
• Sensitivity: 0.24 ng/mL

Porcine Coagulation Factor II/Thrombin ELISA Kit (Colorimetric) Summary

• Description: F2 (Pig) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of pig F2.
• Standard Curve Range: 0.391 to 100 ng/mL
• Sensitivity: 0.24 ng/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 7.4%
• Intra-Assay: CV% < 4.8%
• Spike Recovery: 96%
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: F2

Applications/Dilutions

• Dilutions:
  • ELISA
  • Quantification

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Porcine Coagulation Factor II/Thrombin ELISA Kit (Colorimetric)

• coagulation factor II (thrombin) receptor-like 2
• Coagulation factor II receptor-like 2 (protease-actovated receptor 3)
• Coagulation factor II receptor-like 2
• Coagulation Factor II
• F2
• PAR-3
• PAR3proteinase-activated receptor 3
• protease-activated receptor 3
• proteinase-activated receptor-3
• PT
• serine protease
• Thrombin receptor-like 2
• Thrombin

Background

Proteinase-activated receptor 3 (PAR3) is a member of the Proteinase-Activated Receptor subfamily. The enzyme thrombin is involved in the activation of platelets, leukocytes, endothelial cells, and mesenchymal cells at sites of vascular injury. These cellular responses are triggered through proteolytic activation of PAR3 and other receptors by thrombin. It is believed that PAR3 functions as a cofactor for the cleavage and activation of PAR4. PAR3 expression has been documented in blood, bone marrow, lung, spleen, and vessel. ESTs have been isolated from head/neck, skeletal muscle, and skin libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Coagulation Factor II/Thrombin ELISA Kit (KA1407)(1)

Publication using KA1407

• Tsai HC, Chang GR, Fan HC et al. A mini-pig model for evaluating the efficacy of autologous platelet patches on induced acute full thickness wound healing BMC Vet. Res. 2019-06-07 [PMID: 31174527] (ELISA, Porcine)

FAQs for Coagulation Factor II/Thrombin ELISA Kit (KA1407). (Showing 1 - 2 of 2 FAQ).

1. Do you have neutralizing antibodies for anti-mouse Thrombin?
   • We currently have 1 neutralizing Thrombin antibody (NBP1-95029), however it is an anti-human antibody and has only been validated in human samples. If you would like to test this antibody in mouse samples, you may be interested in participating in our Innovators Reward Program.
2. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: F2