Coagulation Factor II/Thrombin Antibody (8.H.11)

Immunohistochemistry-Paraffin: Prothrombin Antibody (8.H.11) [NBP1-40254] - Analysis of anti-F2 / Thrombin antibody with human liver at concentration 5 ug/ml.

Product Discontinued

Coagulation Factor II/Thrombin Antibody (8.H.11) Summary

• Immunogen: Human Coagulation Factor II/Thrombin
• Specificity: Recognizes human Prothrombin, prethrombin-1, fragment 2, meizothrombin. Inhibits clotting and prothrombin activation.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: F2
• Purity: Ion exchange chromatography

Applications/Dilutions

• Dilutions:
  • ELISA 5 - 10 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 10 ug/ml

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Distilled water
• Preservative: 50% glycerol
• Concentration: 1.0 mg/ml
• Purity: Ion exchange chromatography

Alternate Names for Coagulation Factor II/Thrombin Antibody (8.H.11)

• coagulation factor II (thrombin) receptor-like 2
• Coagulation factor II receptor-like 2 (protease-actovated receptor 3)
• Coagulation factor II receptor-like 2
• Coagulation Factor II
• F2
• PAR-3
• PAR3proteinase-activated receptor 3
• protease-activated receptor 3
• proteinase-activated receptor-3
• PT
• serine protease
• Thrombin receptor-like 2
• Thrombin

Background

Coagulation factor II is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. F2 also plays a role in maintaining vascular integrity during development and postnatal life. Mutations in F2 leads to various forms of thrombosis and dysprothrombinemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Coagulation Factor II/Thrombin Antibody (NBP1-40254). (Showing 1 - 2 of 2 FAQ).

1. Do you have neutralizing antibodies for anti-mouse Thrombin?
   • We currently have 1 neutralizing Thrombin antibody (NBP1-95029), however it is an anti-human antibody and has only been validated in human samples. If you would like to test this antibody in mouse samples, you may be interested in participating in our Innovators Reward Program.
2. I wanted to know if there is any epitope information for this antibody?
   • This antibody is raised against full-length human prothrombin, and unfortunately the epitope has not been mapped at this time. The antibody has been tested and found to detect human prothrombin, prethrombin-1, fragment 2, and meizothrombin, but this is unfortunately the only information available regarding specificity.

Bioinformatics

• Gene Symbol: F2
• Entrez:
  • 2147(Human)
• Uniprot:
  • P00734()