CNOT4 Antibody (OTI1H2)

Western Blot: CNOT4 Antibody (1H2) [NBP2-03878] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CNOT4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CNOT4.

Immunohistochemistry-Paraffin: CNOT4 Antibody (1H2) [NBP2-03878] Staining of paraffin-embedded Human tonsil using anti-CNOT4 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: CNOT4 Antibody (1H2) [NBP2-03878] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-CNOT4 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: CNOT4 Antibody (1H2) [NBP2-03878] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CNOT4 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: CNOT4 Antibody (1H2) [NBP2-03878] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-CNOT4 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: CNOT4 Antibody (1H2) [NBP2-03878] - Staining of paraffin-embedded Human endometrium tissue using anti-CNOT4 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: CNOT4 Antibody (1H2) [NBP2-03878] - Staining of paraffin-embedded Human prostate tissue using anti-CNOT4 mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: OTI1H2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

CNOT4 Antibody (OTI1H2) Summary

• Description: Novus Biologicals Mouse CNOT4 Antibody (OTI1H2) (NBP2-03878) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human recombinant protein fragment corresponding to amino acids 190-455 of human CNOT4 (NP_037448) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CNOT4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
• Theoretical MW: 70.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CNOT4 Antibody (OTI1H2)

• CCR4-associated factor 4
• CCR4-NOT transcription complex, subunit 4
• E3 ubiquitin-protein ligase CNOT4
• EC 6.3.2.-
• NOT4yeast) homolog

Background

The protein encoded by this gene is a subunit of the CCR4-NOT complex, a global transcriptional regulator. The encoded protein interacts with CNOT1 and has E3 ubiquitin ligase activity. Several transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CNOT4
• Entrez:
  • 4850(Human)