CNOT3 Antibody

Western Blot: CNOT3 Antibody [NBP1-21396] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NBP1-21396 used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate

Immunohistochemistry-Paraffin: CNOT3 Antibody [NBP1-21396] - Human testicular seminoma. Antibody used at a dilution of 1:200 (1ug/ml).

Western Blot: CNOT3 Antibody [NBP1-21396] - Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, and mouse TCMK-1 cells.

Western Blot: CNOT3 Antibody [NBP1-21396] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells.

Immunohistochemistry-Paraffin: CNOT3 Antibody [NBP1-21396] - of human ovarian carcinoma. Dilution of 1:1,000 (1ug/ml).

Immunohistochemistry-Paraffin: CNOT3 Antibody [NBP1-21396] - FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-CNOT3 used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

CNOT3 Antibody Summary

• Description: Novus Biologicals Rabbit CNOT3 Antibody (NBP1-21396) is a polyclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 525 and 575 of human CCR4-NOT transcription complex, subunit 3 using the numbering given in entry NP_055331.1 (GeneID 4849).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CNOT3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CNOT3 Antibody

• CCR4-NOT transcription complex, subunit 3
• KIAA0691CCR4-associated factor 3
• LENG2yeast) homolog
• Leukocyte receptor cluster member 2

Background

CNOT3 (CCR4-NOT transcription complex subunit 3) is a component of the CCR4-NOT core complex that functions as a general transcriptional regulator. In a genome-wide siRNA screen for genes essential for mouse embryonic stem cell self-renewal, CNOT3 was identified and found to co-occupy gene promoters with c-Myc and Zfx.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CNOT3
• Entrez:
  • 4849(Human)
• Uniprot:
  • O75175(Human)