CNAP1 Antibody (5E0Q9)

Western Blot: CNAP1 Antibody (5E0Q9) [NBP3-33550] - Western blot analysis of lysates from HeLa cells, using at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.

Western Blot: CNAP1 Antibody (5E0Q9) [NBP3-33550] - Western blot analysis of various lysates, using at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 180s.

• Reactivity: Hu, Mu
• Applications: WB, ELISA
• Clone: 5E0Q9
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

CNAP1 Antibody (5E0Q9) Summary

• Description: Novus Biologicals Rabbit CNAP1 Antibody (5E0Q9) (NBP3-33550) is a monoclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 100-200 of human CNAP1 (Q15021).
  
  Sequence:
  LDDTTLSGSDRNAHLNALKMNCYALIRLLESFETMASQTNLVDLDLGGKGKKARTKAAHGFDWEEERQPILQLLTQLLQLDIRHLWNHSIIEEEFVSLVTG
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: NCAPD2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA Recommended starting concentration is 1 ug/mL
  • Western Blot 1:500 - 1:1000
• Theoretical MW: 157 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for CNAP1 Antibody (5E0Q9)

• CAPD2
• CAP-D2
• Chromosome condensation-related SMC-associated protein 1
• Chromosome-associated protein D2
• CNAP1XCAP-D2 homolog
• hCAP-D2condensin complex subunit 1
• KIAA0159chromosome condensation related SMC associated protein 1
• Non-SMC condensin I complex subunit D2
• non-SMC condensin I complex, subunit D2

Background

CNAP1/hCAP-D2 is a regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condensed chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. It may target the condensin complex to DNA via its C-terminal domain (referenced from Swissprot).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NCAPD2