CMYA5 Antibody - BSA Free

Western Blot: CMYA5 Antibody [NBP1-77117] - Analysis in mouse heart tissue lysate with antibody at 1 ug/mL.

Immunohistochemistry: CMYA5 Antibody [NBP1-77117] - SPRYD2 in mouse brain tissue with SPRYD2 antibody at 20 ug/mL.

Immunohistochemistry: CMYA5 Antibody [NBP1-77117] - SPRYD2 in mouse brain tissue with SPRYD2 antibody at 2.5 ug/mL.

Immunohistochemistry: CMYA5 Antibody - BSA Free [NBP1-77117] - Immunohistochemistry of CMYA5 in mouse brain tissue with CMYA5 antibody at 2.5 u/mL.

Immunocytochemistry/ Immunofluorescence: CMYA5 Antibody - BSA Free [NBP1-77117] - Immunofluorescence of CMYA5 in mouse brain tissue with CMYA5 antibody at 20 u/mL.

Immunohistochemistry: CMYA5 Antibody - BSA Free [NBP1-77117] - Immunohistochemistry of CMYA5 in mouse brain tissue with CMYA5 antibody at 2.5 u/mL.

Immunocytochemistry/ Immunofluorescence: CMYA5 Antibody - BSA Free [NBP1-77117] - Immunofluorescence of CMYA5 in mouse brain tissue with CMYA5 antibody at 20 u/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

CMYA5 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit CMYA5 Antibody - BSA Free (NBP1-77117) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human SPRYD2. The immunogen is located within amino acids 3810 - 3860 of SPRYD2. Amino Acid Squence: TIRWRPTTPEATETYTLE
• Specificity: SPRYD2 antibody is predicted to not cross-react with other SPRYD protein family members. At least four isoforms of SPRYD2 are known to exist.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CMYA5
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 2.5 ug/mL
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 1-2 ug/ml
• Control Peptide:

  CMYA5 Peptide (NBP1-77117PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for CMYA5 Antibody - BSA Free

• C5orf10
• cardiomyopathy associated 5
• cardiomyopathy-associated protein 5
• DKFZp451G182
• DKFZp451G223
• DTNBP2
• dystrobrevin-binding protein 2
• genethonin-3
• myospryn
• SPRY domain-containing protein 2
• SPRYD2
• tripartite motif-containing protein 76

Background

SPRYD2, also known as Myospryn, was originally identified as the muscle-specific partner of dysbindin and as a Mef-2 target gene. It is a large scaffolding protein localized to the Z-disc/costamere region of striated muscle. SPRYD2 includes a noncanonical tripartite motif (TRIM-like) that lacks the RING domain but consists of a B-box coiled coil (BBC), fibronectin 3 (FN3) repeats, and SPRY domains. SPRYD2 interacts with desmin and calcineurin, and it has been suggested to play a role in the biogenesis of lysosome and negatively regulates slow-fiber-type transformation and skeletal muscle regeneration. SPRYD2 is dysregulated in Duchenne muscular dystrophy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CMYA5
• Uniprot:
  • NP_705838(Human)