CMYA5 Antibody

Images

 
Western Blot: CMYA5 Antibody [NBP1-77117] - Analysis in mouse heart tissue lysate with antibody at 1 ug/mL.
Immunocytochemistry/ Immunofluorescence: CMYA5 Antibody [NBP1-77117] - SPRYD2 in mouse brain tissue with SPRYD2 antibody at 20 ug/mL.
Immunohistochemistry: CMYA5 Antibody [NBP1-77117] - SPRYD2 in mouse brain tissue with SPRYD2 antibody at 2.5 ug/mL.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

CMYA5 Antibody Summary

Immunogen
Antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human SPRYD2. The immunogen is located within amino acids 3810 - 3860 of SPRYD2.
Specificity
SPRYD2 antibody is predicted to not cross-react with other SPRYD protein family members. At least four isoforms of SPRYD2 are known to exist.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CMYA5
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/mL.
  • Immunohistochemistry 2.5 ug/mL
  • Immunofluorescence
Control Peptide
CMYA5 Peptide (NBP1-77117PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CMYA5 Antibody

  • C5orf10
  • cardiomyopathy associated 5
  • cardiomyopathy-associated protein 5
  • DKFZp451G182
  • DKFZp451G223
  • DTNBP2
  • dystrobrevin-binding protein 2
  • genethonin-3
  • myospryn
  • SPRY domain-containing protein 2
  • SPRYD2
  • tripartite motif-containing protein 76

Background

SPRYD2, also known as Myospryn, was originally identified as the muscle-specific partner of dysbindin and as a Mef-2 target gene. It is a large scaffolding protein localized to the Z-disc/costamere region of striated muscle. SPRYD2 includes a noncanonical tripartite motif (TRIM-like) that lacks the RING domain but consists of a B-box coiled coil (BBC), fibronectin 3 (FN3) repeats, and SPRY domains. SPRYD2 interacts with desmin and calcineurin, and it has been suggested to play a role in the biogenesis of lysosome and negatively regulates slow-fiber-type transformation and skeletal muscle regeneration. SPRYD2 is dysregulated in Duchenne muscular dystrophy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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CMYA5 NBP1-77117

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Bioinformatics

Gene Symbol CMYA5
Uniprot