CMP kinase Overexpression Lysate

Western Blot: CMP kinase Overexpression Lysate (Adult Normal) [NBL1-09298] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CMP kinase.

• Reactivity: Hu
• Applications: WB

CMP kinase Overexpression Lysate Summary

• Description:

  CMP kinase Transient Overexpression Lysate

  
  Expression Host: HEK293T
  
  Plasmid: RC204856
  
  Accession#: NM_016308
  
  Protein Tag: C-MYC/DDK
• Gene: CMPK1

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This product is intended for use as a positive control in Western Blot.
  
  You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
• Theoretical MW: 25.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for CMP kinase Overexpression Lysate

• CMKEC 2.7.4.14
• CMPKUMKUMP-CMP kinase
• cytidine monophosphate (UMP-CMP) kinase 1, cytosolic
• Cytidine monophosphate kinase
• Cytidylate kinaseUMP/CMP kinase
• Deoxycytidylate kinase
• EC 2.7.4
• UMP/CMPK
• UMP-CMPK
• UMPKUCK
• Uridine monophosphate kinase
• Uridine monophosphate/cytidine monophosphate kinase

Background

Uridine monophosphate (UMP)/cytidine monophosphate (CMP) kinase (EC 2.7.4.4) catalyzes the phosphoryl transfer from ATP to UMP, CMP, and deoxy-CMP (dCMP), resulting in the formation of ADP and the corresponding nucleoside diphosphate. These nucleoside diphosphates are required for cellular nucleic acid synthesis (Liou et al., 2002 [PubMed 11912132]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: CMPK1