CMBL Antibody (2B11) - BSA Free

Western Blot: CMBL Antibody (2B11) [NBP2-22618] - The extracts of HeLa (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CMBL antibody (1:500 ~ 1:5000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 2B11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

CMBL Antibody (2B11) - BSA Free Summary

• Description: Novus Biologicals Mouse CMBL Antibody (2B11) - BSA Free (NBP2-22618) is a monoclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant human CMBL (1-245aa) purified from E. coli
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CMBL
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Western Blot 1:500-1:5000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for CMBL Antibody (2B11) - BSA Free

• carboxymethylenebutenolidase homolog (Pseudomonas)
• carboxymethylenebutenolidase homolog
• carboxymethylenebutenolidase-like (Pseudomonas)
• EC 3.1
• FLJ23617

Background

CMBL(carboxymethylenebutenolidase homolog) is a 245 amino acid cytosolic protein and cysteine hydrolase of the dienelactone hydrolase family that is highly expressed in liver cytosol. CMBL is the human homolog of Pseudomonas dienelactone hydrolase, a protein that participates in the bacterial halocatechol degradation pathway. That is ubiquitously expressed with high expression in liver, colon, kidney and small intestine, where it acts as a bioactivating enzyme for a prodrug type angiotensin II type 1 receptor antagonist known as olmesartan medoxomil. CMBL is inhibited by p-chloromercuribenzoate (PCMB).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CMBL
• Uniprot:
  • NP_620164(Human)